Larisa Pereboeva, M.D., Ph.D.
Instructor
Division of Human Gene Therapy
Department of Pathology

901 19th Street South
BMR II - 572
Birmingham, AL 35294-2172

Email: larisa.pereboeva@ccc.uab.edu
Phone: (205) 975-8734


Research Interests

We are interested in developing a cell-based delivery of anticancer therapeutics to tumors. Cell vectors offer a possibility to utilize autologous cells and to reduce toxicity caused by injection of high dose of viral vectors or anticancer drugs. Specifically, we employ mesenchymal stem/progenitor cells (MPCs) as a means to deliver suicide/therapeutic/ genes as well as oncolytic viruses. Recently these cells have gained a great interest in cell therapy for bone and cartilage disorders as well as for the transplantation studies. MPCs represent a good candidate as a cellular vector due to their favorable intrinsic properties, such as simple isolation and culturing, ability to be expanded in vitro to therapeutic quantities, ability to secrete therapeutic proteins. In addition, growing body of data has documented an interesting property of this cell population to home to the sites of tissue injury or tissue stress. Importantly, it has been demonstrated that MPC after systemic injection can preferentially engraft to solid tumor and participate in the formation of tumor stroma. Thus, this cell population is considered as a very attractive candidate for the cell-based delivery in cancer settings. Our major efforts concentrated on testing feasibility and efficacy of using these cells as vehicles and investigating advantages of cell-based delivery of therapeutics. Another area of investigation is to influence homing of cell vehicles to tumors.

We also work on genetic modification of adenoviral vectors by constructing fiber-mosaic adenoviruses for expanding virus tropism or introducing novel properties to adenoviral particles.


Select List of Publications


  1. Pereboeva L, Komarova S, Roth J, Ponnazhagan S, Curiel DT. Targeting EGFR with metabolically biotinylated fiber-mosaic adenovirus. Gene Ther. 2007 Jan 25

  2. Stoff-Khalili MA, Rivera AA, Mathis JM, Banerjee NS, Moon AS, Hess A, Rocconi RP, Numnum TM, Everts M, Chow LT, Douglas JT, Siegal GP, Zhu ZB, Bender HG, Dall P, Stoff A, Pereboeva L, Curiel DT. Mesenchymal stem cells as a vehicle for targeted delivery of CRAds to lung metastases of breast carcinoma. Breast Cancer Res Treat. 2007 Jan 13

  3. Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L. Mesenchymal progenitor cells as cellular vehicles for delivery of oncolytic adenoviruses. Mol Cancer Ther. 2006 Mar;5(3):755-66.

  4. Tsuruta Y, Pereboeva L, Glasgow JN, Luongo CL, Komarova S, Kawakami Y, Curiel DT. Reovirus sigma1 fiber incorporated into adenovirus serotype 5 enhances infectivity via a CAR-independent pathway. Biochem Biophys Res Commun. 2005 Sep 16;335(1):205-14.

  5. Stoff-Khalili MA, Rivera AA, Glasgow JN, Le LP, Stoff A, Everts M, Tsuruta Y, Kawakami Y, Bauerschmitz GJ, Mathis JM, Pereboeva L, Seigal GP, Dall P, Curiel DT. A human adenoviral vector with a chimeric fiber from canine adenovirus type 1 results in novel expanded tropism for cancer gene therapy. Gene Ther. 2005 Dec;12(23):1696-706.

  6. Pereboeva L, Curiel DT. Cellular vehicles for cancer gene therapy: current status and future potential. BioDrugs. 2004;18(6):361-85. Review.

  7. Pereboeva L, Komarova S, Mahasreshti PJ, Curiel DT. Fiber-mosaic adenovirus as a novel approach to design genetically modified adenoviral vectors. Virus Res. 2004 Sep 15;105(1):35-46.

  8. Pereboeva, L., Komarova, S., Mikheeva, G., Krasnykh, V., and Curiel, D. T. Approaches to utilize mesenchymal progenitor cells as cellular vehicles. Stem Cells (2003) 21: 389-404.

  9. Pereboev A, Pereboeva L, Curiel DT. Phage display of adenovirus type 5 fiber knob as a tool for specific ligand selection and validation. J Virol (2001)75(15):7107-13.

  10. Pereboeva LA, Pereboev AV, Wang LF, Morris GE. Hepatitis C epitopes from phage-displayed cDNA libraries and improved diagnosis with a chimeric antigen. J Med Virol (2000)60(2):144-5.

  11. Pereboeva LA, Pereboev AV, Morris GE. Identification of antigenic sites on three hepatitis C virus proteins using phage-displayed peptide libraries. J Med Virol (1998)56(2):105-11.

  12. Kanev AN, Vorob'eva MS, Shalunova NV, Pereboeva LA, Maksiutov AZ, Kiselev NN, Shalaev EIu, Iastrebova ON, Varaksin NA. Constructing the reference panel of sera with standard level of IgG-antibodies. Vopr Virusol 1996 Jul-Aug;41(4):161-6. [Article in Russian]

  13. Ryabchikova E, Kolesnikova L, Smolina M, Tkachev V, Pereboeva L, Baranova S, Grazhdantseva A, Rassadkin Y. Ebola virus infection in guinea pigs: presumable role of granulomatous inflammation in pathogenesis. Arch Virol 1996;141(5):909-21.


Biosketch

Current Positions

1997 - Present Research Assistant/Research Associate/instructor UAB, Gene Therapy Center, Division of Human Gene Therapy

Previous Research Appointments

1985 - 1993 Junior Research Scientist/Research Scientist, Laboratory of Bioanalysis and Laboratory of Electron Microscopy, Department of Molecular Virology, Institute of Molecular Biology, Scientific-Production Association "VECTOR", Koltsovo, Novosibirsk region, Russia
1993 - 1995 Senior Scientist, Joint-Stock Company "Vector-Best", Koltsovo, Novosibirsk region, Russia
1995 - 1997 PhD student, MRIC Biochemistry Group, the North East Wales Institute (NEWI), Wrexham, UK.

Academic Degrees

1985 MD in Biochemistry
1999 PhD in Virology