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The Lister Hill Library of the Health Sciences, established in 1945, is the largest biomedical library in Alabama and one of the leading such libraries in the South. It serves as a Resource Library in the National Network of Libraries of Medicine for the Southeast/Atlantic region.

Its collections span seven centuries of knowledge beginning with the 30 incunabula within the thirteen thousand old and rare books to thousands of electronic subscriptions through both individual publisher arrangements, aggregated packages, and consortial agreements. The volumes of books, bound journals, and other media currently held total approximately 334,000 volumes.