PI: Joao DeAndrade, MD

Director: Dr. Lisa Schwiebert, PhD

Contact: Tonja Meadows, RN 205-934-7630

IRB approved facility offers LHC members access to samples derived from multiple lung diseases (transplant, idiopathic pulmonary fibrosis, chronic obstructive pulmonary disease and asthma)

Applications for pulmonary biospecimens include, but are not limited to analyses of immune cell trafficking and signal transduction as well as inflammatory mediator expression.

Collection, storage, and assessment:  

Bronchoalveolar lavage (BAL) samples. Collected BAL samples are centrifuged to pellet the cellular fraction. Cell are counted and differentiated on cytospin preps using Wright-Giemsa stain. Upon request, cell differentials are then determined from at least 500 leukocytes using standard hematological criteria. Remaining cells are stored as cell pellets at -80oC for RNA, DNA, or protein analyses. Supernatants are stored at -80oC until distribution

Plasma samples. Collected blood samples are ficolled and cells are harvested from buffy coat layers. Cells are then counted and differentiated on cytospin preps using Wright-Giemsa stain. Upon request, cell differentials are then determined from at least 500 leukocytes using standard hematological criteria. Remaining cells are stored as cell pellets at -80C for RNA, DNA, or protein analyses. Plasma fluid samples are stored at -80oC until distribution.

Serum samples. Collected blood samples are centrifuged and the serum is aliquoted and stored at -80oC until distribution.

Sample organization and quality control. All samples are logged into the PBR using FreezerWorks, a state-of-the-art cataloging program designed for the organization of stored clinical samples. Cell and fluid samples are assessed routinely for viability.

Patient clinical history. Each sample is linked directly with the respective patient clinical history via P-TREC, a HIPPA compliant comprehensive clinical database developed by the Division of Pulmonary, Allergy and Critical Care Medicine and hosted by the Division of Preventive Medicine.

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