Welcome to Targeted Metabolomics and Proteomics Laboratory

Prostaglandins in
wild-type and Cox
knockout mouse
Neurosensory perception of environmental cues modulates sperm motility critical for fertilization

Katherine McKnight, Hieu D. Hoang, Jeevan K. Prasain, Naoko Brown, Jack Vibbert, Kyle A. Hollister, Ray Moore, Justin R. Ragains, Jeff Reese, and Michael A. Miller*

Oocytes of C. elegans secrete novel F class prostaglandins that attract sperm toward them. The prostaglandins were identified by LC-MSMS studies conducted in TMPL by Dr. Jeevan Prasain. They are also synthesized in Cox knockout mice, raising the possibility that similar mechanisms exist in other animals.

Science. 2014;344(6185):754-7.
[doi: 10.1126/science.1250598] [PMID: 24833393] [PMCID: PMC4094289]

2nd Annual Workshop on Metabolomics

June 2-5, 2014

The 2nd Annual Workshop on Metabolomics was held June 2-5 2014 on the campus of the University of Alabama at Birmingham.

The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Department of Pharmacology and Toxicology at UAB.

Go to Metabolomics Workshop web page.

Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
[PMID: 20346741]
TMPL Movie
PPT file

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About TMPL

The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has four mass spectrometers, two triple quadrupole instruments (AB Sciex 3200 and 4000), a quadrupole-linear ion trap instrument (AB Sciex 4000Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The recent addition of an Eksigent microflow 200 LC provides significant improvement in sensitivity for the triple quadrupole instruments.

Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (renfrow@uab.edu; 205 996-4681).

A Bruker MALDI TOF-TOF instrument is available in the Comprehensive Cancer Center Core Laboratory of Dr. Jim Mobley (Jim.Mobley@ccc.uab.edu; 205 996-6363).

New sample and data-handling arrangements in TMPL – investigators please note

Latest publication

Wilson L, Arabshahi A, Simons B, Prasain JK, Barnes S.
Improved high sensitivity analysis of polyphenols and their metabolites by nano-liquid chromatography-mass spectrometry.
Arch Biochem Biophys. 2014 Oct 1;559:3-11. [doi: 10.1016/j.abb.2014.06.014] [PMID: 24967696]

See full publication list.