Welcome to Targeted Metabolomics and Proteomics Laboratory

UAB Tissue Imaging Mass Spectrometry detects early lipid changes in acute kidney injury

by Jeff Hansen

Janusz Kabarowski and SteveBarnes

University of Alabama at Birmingham researchers have made a microscopic snapshot of the early renal lipid changes in acute kidney injury, using a laser-scanning method called MALDI tissue imaging to localize the changes.

These disease-model results, recently published in American Journal of Physiology’s Renal Physiology, show an example of the power of MALDI tissue imaging. MALDI tissue imaging is now available at UAB, and it will be able to aid basic and clinical biomedical research across the campus, said corresponding author Janusz Kabarowski, Ph.D., associate professor of microbiology.

Read more at UAB News.

Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice. [PMID: 26466956]

In this paper from the laboratory of Dr. Janusz Kabarowski in the Department of Microbiology, the TMPL group worked with Dr. Kabarowski to develop an LC-MS/MS assay for major unsaturated fatty acids like linoleic and arachidonic acids, and their oxidized metabolites called hydroxyoctadecadienoic and hydroxyeicosatetraenoic acids (HODEs and HETEs respectively) in a study designed to test anti-inflammatory properties of HDL in Lupus. Transfer of Lupus disease by bone marrow transplantation into mice engineered to have high HDL levels resulted in immune suppression that was associated with elevations in immune cell content of 9-HODE and 13-HODE, whereas cholesterol levels were unaffected. 9-HODE and 13-HODE are potent natural activators of the anti-inflammatory nuclear receptor, peroxisome proliferator-activated receptor-γ (PPAR-γ), and as such may constitute a lipid pathway in Lupus counterbalancing immune hyperactivation and thus mitigating clinical sequelae such as Lupus nephritis.

See full publication list.

4th Annual Workshop on Metabolomics

July 17-21, 2016

The 4th Annual Workshop on Metabolomics will be held July 17-21, 2016 on the campus of the University of Alabama at Birmingham.

The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Departments of Chemistry and Pharmacology and Toxicology at UAB.

More details on the workshop and how to register will appear shortly on the Metabolomics Workshop web page.


MRM
Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
[PMID: 20346741]
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About TMPL

The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.

Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (renfrow@uab.edu; 205 996-4681).

A Bruker MALDI TOF-TOF instrument is available in the Comprehensive Cancer Center Core Laboratory of Dr. Jim Mobley (mobleyja@uab.edu; 205 996-6363).

New sample and data-handling arrangements in TMPL – investigators please note