Welcome to Targeted Metabolomics and Proteomics Laboratory
Exciting cross departmental science involving TMPL(UAB News, Jan 28, 2014)
TMPL has supported an interdepartmental research project typical of the collaboration that can occur at UAB. Miranda Collier, an honors student in the Department of Chemistry, who has worked for the past four years in Dr. Stephen Barnes’ laboratory studying the biochemistry of the lens of zebrafish, teamed up with Dr. Janusz Kabarowski (Microbiology), Dr. Stephen Watts (Biology) and Dr. David Graves (Chemistry) to develop an imaging mass spectrometry method for the distribution of lipids in thin frozen sections of lens of the zebrafish. This technique has great promise for application to a wide range of applications in biological systems across the campus. The TMPL group applied lipidomics analysis of extracts of the lens using the AB Sciex 5600TripleTOF mass spectrometer to identify the principal lipids of the lens.
2nd Annual Workshop on MetabolomicsJune 2-5, 2014
The 2nd Annual Workshop on Metabolomics was held June 2-5 2014 on the campus of the University of Alabama at Birmingham.
The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Department of Pharmacology and Toxicology at UAB.
|Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has four mass spectrometers, two triple quadrupole instruments (AB Sciex 3200 and 4000), a quadrupole-linear ion trap instrument (AB Sciex 4000Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The recent addition of an Eksigent microflow 200 LC provides significant improvement in sensitivity for the triple quadrupole instruments.
Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (firstname.lastname@example.org; 205 996-4681).