Welcome to Targeted Metabolomics and Proteomics Laboratory
Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice. [PMID: 26466956]
In this paper from the laboratory of Dr. Janusz Kabarowski in the Department of Microbiology, the TMPL group worked with Dr. Kabarowski to develop an LC-MS/MS assay for major unsaturated fatty acids like linoleic and arachidonic acids, and their oxidized metabolites called hydroxyoctadecadienoic and hydroxyeicosatetraenoic acids (HODEs and HETEs respectively) in a study designed to test anti-inflammatory properties of HDL in Lupus. Transfer of Lupus disease by bone marrow transplantation into mice engineered to have high HDL levels resulted in immune suppression that was associated with elevations in immune cell content of 9-HODE and 13-HODE, whereas cholesterol levels were unaffected. 9-HODE and 13-HODE are potent natural activators of the anti-inflammatory nuclear receptor, peroxisome proliferator-activated receptor-γ (PPAR-γ), and as such may constitute a lipid pathway in Lupus counterbalancing immune hyperactivation and thus mitigating clinical sequelae such as Lupus nephritis.
4th Annual Workshop on Metabolomics
July 17-21, 2016
The 4th Annual Workshop on Metabolomics will be held July 17-21, 2016 on the campus of the University of Alabama at Birmingham.
The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Departments of Chemistry and Pharmacology and Toxicology at UAB.
More details on the workshop and how to register will appear shortly on the Metabolomics Workshop web page.
|Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.
Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (email@example.com; 205 996-4681).