• Cryo-Electron Microscopy Core
    Cryo-Electron Microscopy Core

    Director: Terje Dokland
    Email: dokland@uab.edu
    Phone: 996-4502

    Contact
    Cindy Rodenburg
    cindyr@uab.edu
    205-996-4510

    The cryo-electron microscopy facility provides capabilities for high resolution electron microscopy and tomography of stained and unstained specimens. It is equipped with an FEI Tecnai F20 cryo-electron microscope. Cryo-EM allows the observation of biological samples in their native environment, in the absence of the distortions and artifacts associated with traditional sample preparation methods. The facility also has a Leica UC6 cryo-microtome for ultrathin sectioning of frozen samples. The instruments are available for use by trained users (training is available) or as a core service.

    Equipment

    • FEI Tecnai F20 200kV FEG electron microscope with Gatan Ultrascan 4000 CCD camera
    • Gatan 626 cryo-sample holder
      FEI Vitrobot sample vitrification apparatus
    • Leica Ultracut UC6 cryo-microtome
    • Emitech K100X glow discharge apparatus

    Center Affiliation
    Center for Biophysical Sciences and Engineering

    Serves - UAB Internal and External