• Central Alabama High Field NMR Facility
    Central Alabama High Field NMR Facility

    Director: N  Rama Krishna
    Email: nrk@uab.edu
    Phone: 205 934-5695

    Contact
    Ronald Shin, Ph.D.
    shinr@uab.edu
    205-934-5696
    The Facility Staff will be happy to collect data as a service, or allow NMR-trained users to collect the data themselves. The staff provides advice
    and consultation to users in the following Research Capabilities useful In Structural Biology and Drug Design and Discovery Efforts:
    1. Quality Control ln Synthetic Chemistry and Medicinal Chemistry Drug Discovery/Design Projects by 1D/2D-NMR (1H, 13C, 19F, 31P, 195Pt. etc).
    2. Determination of the three-dimensional structures of proteins and their complexes (protein-protein, protein-nucleic acid, protein-carbohydrate).
    3. Protein complexes with low molecular weight compounds (including low-affinity compounds). The bound·structure of the compound can be used in Structure-based design of improved lead compounds and ultimately drugs.
    4. STD-NMR Screening (a binding assay) of single Compound or Compound Libraries to identify "hits'' recognized by a target protein. Group epitope mapping of ligands. STD-NMR/CORCEMA-ST analyses of bound-ligand conformations.
    5. Fragment-based discovery & design (lead optimization) of new drugs by: (a) SAR-by-NMR (a technique pioneered by the Abbott Laboratories, widely used in Pharmaceutical Industry), and the (b) ILOE technique
    6. Membrane proteins in solution (proteins in micelles and bicelles).
    7. NMR of cells, cell extracts, and Metabonomlcs-by-NMR

    Equipment
    The Facility provides access to the following stale-of-the-art NMR instrumentation
    1.850 MHz Bruker Avance III NMR Spectrometer (Ascend) with a CryoProbe (installation in progress)
    2.700 MHz Bruker Avance II NMR with a CryoProbe
    3.600 MHz Bruker Avance III NMR Spectrometer with a CryOProbe
    4.500 MHz Bruker Avance NMR Spectrometer with TXI and TBI probes

    Center Affiliations
    Center for Biophysical Sciences and Engineering
    Comprehensive Cancer Center

    Grant Affiliations
    NCI CA-13148 (Cancer Center Support Grant)

    Serving - UAB Internal and External