• IDDRC Core C: Neurobiology Imaging and Tissue Processing
    IDDRC Core C: Neurobiology Imaging and Tissue Processing

    Director: Lucas Pozzo-Miller, Ph.D.
    Email: lucaspm@uab.edu
    Phone: 205-975-4659

    Website -  http://www.mrrc.uab.edu/default.htm

    Contact
    Alison Margolies
    kamargo@uab.edu
    205-934-0743

    Shelby 1062:

    Prairie 2-Photon Microscope with 2 independent mode-locked Ti:Sapphire lasers, 4 sensitive GaAsP PMTs for broadband epi- and trans-fluorescence imaging. Users image tissue slices, or perform in vivo imaging, like brain imaging through thinned skull or craniotomy windows.

    Zeiss Spectral 510-META Confocal on a Zeiss Axioskop2 wide field fluorescence microscope equipped with a tunable argon laser (458, 477, 488, 514nm) and two HeNe lasers (543 and 633 nm), 2 PMTs and 1 META detector allowing for the discrimination between fluorophores with closely spaced or overlapping emission spectra with the “Meta” detector.

     

    Shelby 130:

    Tissue processing laboratory equipped with perfusion set-up for small rodents (rats and mice), sectioning areas, chemical bench, balances, centrifuges, embedding vacuum ovens, compound light microscope, and staining set-ups.

    Pelco 3450 Microwave Processor for enhanced aldehyde fixation using microwaves and feedback, liquid-cooling temperature control during fixation, staining, dehydration, and pre-embedding for LM and TEM.

     Microm Cryo-Star HM560V cryostat for obtaining frozen semi-thin sections. An electronic, motorized cryostat with retraction that incorporates a unique refrigeration system for independent specimen and knife cooling.

     Zeiss AxioImager upright brightfield and/or fluorescence microscope. Filters include: DAPI, GFP, CY3/Rhodamine, and Texas Red. Dry and oil-immersion objectives.  Equipped with a 5 megapixel Zeiss mRc5 camera along with Zeiss AxioVision software, and a Dell personal computer.

    Stereo Investigator software that is designed to help obtain the most efficient, precise, and unbiased estimate of cell populations, as well as morphometric properties of biological structures.

    Tissue processing for TEM. All TEM Imaging is done at UAB High Resolution Imaging Facility: http://main.uab.edu/show.asp?durki=34926

     

    IDDRC Core C provides training and assistance to IDDRC investigators and their lab personnel. Assistance is offered on an as-needed basis and investigators are allowed to run the microscope by themselves after training. A sign-up scheduling system is available on the website for Core C microscopes. There are no service fees for the use of the Core. Interested investigators wishing to become IDDRC investigators to gain access to all its Cores should send their request to the IDDRC Director: Dr. Alan Percy apercy@uab.edu

     

    Contact Information:
    Core C Director: Lucas Pozzo-Miller, PhD.  lucaspm@uab.edu

    Technical Staff:
    Tong Ye, PhD:  TYe@nrc.uab.edu
    Alison Margolies: margo@nrc.uab.edu

     

    Equipment
    Shelby 1062:
    Prairie 2-Photon Microscope with 2 independent mode-locked Ti:Sapphire lasers, 4 sensitive GaAsP PMTs for broadband epi- and trans-fluorescence imaging. Users image tissue slices, or perform in vivo imaging, like brain imaging through thinned skull or craniotomy windows.

    Zeiss Spectral 510-META Confocal on a Zeiss Axioskop2 wide field fluorescence microscope equipped with a tunable argon laser (458, 477, 488, 514nm) and two HeNe lasers (543 and 633 nm), 2 PMTs and 1 META detector allowing for the discrimination between fluorophores with closely spaced or overlapping emission spectra with the “Meta” detector.

    Shelby 130:
    Tissue processing laboratory equipped with perfusion set-up for small rodents (rats and mice), sectioning areas, chemical bench, balances, centrifuges, embedding vacuum ovens, compound light microscope, and staining set-ups.

    Pelco 3450 Microwave Processor for enhanced aldehyde fixation using microwaves and feedback, liquid-cooling temperature control during fixation, staining, dehydration, and pre-embedding for LM and TEM.

    Microm Cryo-Star HM560V cryostat for obtaining frozen semi-thin sections. An electronic, motorized cryostat with retraction that incorporates a unique refrigeration system for independent specimen and knife cooling.

    Zeiss AxioImager upright brightfield and/or fluorescence microscope. Filters include: DAPI, GFP, CY3/Rhodamine, and Texas Red. Dry and oil-immersion objectives. Equipped with a 5 megapixel Zeiss mRc5 camera along with Zeiss AxioVision software, and a Dell personal computer.

    Stereo Investigator software that is designed to help obtain the most efficient, precise, and unbiased estimate of cell populations, as well as morphometric properties of biological structures.

    Tissue processing for TEM. All TEM Imaging is done at UAB High Resolution Imaging Facility: http://main.uab.edu/show.asp?durki=34926

    Center Affiliation
    Civitan International Research Center
    Comprehensive Neuroscience Center

    Grant Affiliation
    P30-HD38985 (NIH-NICHD)

    Serves - Center Members Only