• Skin Disease Research Center (SDRC), Skin Cell Culture Core (SCCC)
    Skin Disease Research Center (SDRC), Skin Cell Culture Core (SCCC)

    Director: Laura Timares
    Email: timares@uab.edu
    Phone: 205-934-7545

    Website - http://www.uab.edu/medicine/dermatology/research/skin-disease-center/skin-culture-core

    Yuko Tsuruta 

    Primary human cell cultures from foreskin tissues provide pure cultures of keratinocytes, melanocytes, fibroblasts and dermal microvascular endothelial cells. We also specialize in generating fibroblast cultures from human skin plug biopsy tissue. Murine keratinocyte and fibroblast cultures are obtained from either C57BL/6 mice from our colony or from the researcher's mutant mouse strain using tissue from 1-3 day old newborn pups. Consultation for isolation of Langerhans cells or dendritic cells from murine or human sources, culture of cell lines is also provided. The Skin Cell Culture Core also provides raft cultures, which can be used to investigate differentiated skin biology and this is done with Dr. Louise Chow's laboratory. In addition, the Core has an Amaxa nucleofector, which efficiently transfects plasmid DNA into a wide variety of cell lines and primary cells. (The Core has only a limited set of Amaxa buffers; researchers will typically need to purchase the specific buffer required for their cell of interest from Amaxa.) The SCCC service includes immortalizing primary murine skin cells from mutant mouse strains. These services are available to all UAB researchers for a small fee and are especially valuable to investigators who would like to enter the field of skin research. Fees are waived for investigators who have SDRC pilot and feasibility grants and reduced for SDRC members. Investigators interested in becoming SDRC members should contact Dr. Craig Elmets, Chair of the Department of Dermatology.

    Amaxa Nucleofector
    Cell Culture Facility

    Serves - UAB Internal Only