Histomorphometry
and Molecular Analyses Core (HMAC)
Director:
Gene P. Siegal, MD, PhD
Department/Center
Association: Pathology/Center for
Metabolic Bone Disease (CMBD)
Established: 2001
The Histomorphometry and Molecular Analyses Core (HMAC) provides
state-of-the-art histological, histomorphometric, and highly sensitive cellular
and tissue molecular probe techniques.
The goal of the facility is to provide specialized analysis of hard
tissues through techniques which have previously been unavailable at the Institution
or in the state.
Facility Description
Located in the Lyons-Harrison
Research Building (LHRB), the Core is funded through an
Research Information
Processing bone tissue requires highly specialized techniques, which
require unique equipment and technical expertise. Light microscopy on frozen, paraffin, ground,
and plastic embedded bone sections; state-of-the-art histomorphometry;
epi-fluorescent microscopy of undecalcified sections; and histochemical
analysis along with expert interpretation are provided. Investigators also have access to
sophisticated techniques such as electron microscopy and image analysis of
bone.
Other services available include special stains, immunohistochemistry,
and flow cytometry. Through arrangements
with other Cores, CMBD investigators have access to technologically advanced
expert assistance in molecular techniques.
This includes the capacity to detect and quantitate vector sequences and
specific endogenous gene products within bone, as well as to provide access to
quantitative RT-PCR, tissue in-situ hybridization, and laser capture microdissection
for molecular analysis of bone cells.
Service Availability and Fees
All services are available at a
modest charge to members of the CMBD. For
a nominal fee, outside work is accepted as time permits.
Contact Information
HMAC Principal Investigator: Gene P. Siegal, MD, PhD
Email: gsiegal@path.uab.edu
Phone: 205-934-6608
Laboratory Director: Kang-Jey
Ho, MD, PhD
Email: kjho@path.uab.edu
Phone: 205-933-8101, ext. 6464
Laboratory Supervisor: Patty
Lott, MS, MLT, HT (ASCP)
Email: patty.lott@ortho.uab.edu
Phone: 205-934-2007
Web Site: http://cmbd.path.uab.edu/Bone%20Histopath.htm
Approved by: Gene P. Siegal, MD, PhD and Director
Date: February 22, 2008
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