Protein mass spectrometry 2008
(BMG/PHR 744)
Course will be held on Tuesdays and Fridays 9 - 11 am, January 4-March 20, 2008 in the McCallum Building, 4th floor conference room (401)
Schedule
| Date | Lecturer | Topic |
|---|---|---|
| Jan 4, Fri | S. Barnes/H. Kim | The world of biomolecules. The proteome, proteomics and other -omics and where to start [PDF File] [Questions] |
| Jan 8, Tue | S. Barnes | Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing [PDF File] [Questions] |
| Jan 11, Fri | M. Renfrow | Mass spectrometry - gas phase transfer and instrumentation - include ETD [PDF File] [Questions] |
| Jan 15, Tu | S. Barnes | Ion fragmentation in mass spectrometry; application to proteomics [PDF File] [Questions] |
| Jan 18, Fri | J. Prasain | Ion Fragmentation and small molecules/metabolomics [PDF File, Questions] |
| Jan 22, Tu | S. Barnes | Separating your samples from the matrix - for the analysis [PDF File] [Questions] |
| Mid-term exam - take-home | ||
| Jan 25, Fri | S. Barnes | Purifying proteins for mass spectrometry analysis [PDF File] [Questions] |
| Jan 29, Tu | C. Crasto | The bioinformatics of the proteome [PDF File] |
| Feb 1, Fri | H. Kim | Protein separation by electrophoresis and other 2D-methods [PDF File] [Questions] |
| Feb 5, Tu | J. Mobley | Designing the proteomics and metabolomics experiment [PDF File] |
| Feb 8, Fri | S. Barnes | Mass spectrometry in qualitative and quantitative burrowing of the proteome [PDF File] [Questions] |
| Feb 12, Tu | J. Prasain | Qualitative and quantitative analysis/method validation in metabolomics [PDF File] [Questions] |
| Feb 15, Fri | P. Prevelige | Mass Spectrometry as a Tool for Studying Protein Structure |
| Feb 19, Tu | P. Prevelige | Study of macromolecular structures - protein complexes |
| Feb 22, Fri | S. Barnes | Enzymology and mass spectrometry [PDF File] [Questions] |
| Feb 26, Tu | M. Renfrow | Analysis of protein-protein interactions by affinity purification and mass spectrometry [PDF File 1, PDF File 2, PDF File 3, PDF File 4] |
| Feb 29, Fri | C-C. Wang | Tissue and body fluid proteomics and mass spectrometry [PDF File] |
| Mar 4, Tu | J. Mobley | Tissue imaging by mass spectrometry |
| Mar 7, Fri | M. Renfrow | Applications of FT-ICR-MS and TOF imaging methodology |
| Mar 11, Tu | J. Novak/M. Renfrow | Mass spectrometry in glycomics research - Application to IgA nephropathy |
| Mar 14, Fri | G. Hernandez | Mass spectrometry and biodefense [PDF File] |
| Mar 17, Tu | H. Kim | Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases |
| Mar 20 | Final report due |