Protein mass spectrometry 2010
(BMG/PHR 744)

The science of proteomics and mass spectrometry

Course will be held on Tuesdays and Fridays 9 - 11 am in the McCallum Building, 4th floor conference room (401)


Date Lecturer Topic
Jan 5, Tu S. Barnes/H. Kim The world of biomolecules. The proteome, proteomics and other –omics and where to start [PDF File] [Questions]
Jan 8, Fri M. Renfrow Mass spectrometry – gas phase transfer and instrumentation – include ETD [PDF File] [Questions]
Jan 12, Tu S. Barnes Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing [PDF File] [Questions]
Jan 15, Fri S. Barnes Ion fragmentation in mass spectrometry; application to proteomics [PDF File] [Questions]
Jan 19, Tu S. Barnes Isotopes in mass spectrometry [PDF File] [Questions]
Jan 22, Fri J. Prasain Ion fragmentation of small molecules; Lipidomics [PDF File] [Questions]
Jan 26, Tu S. Barnes/
S. Asmellash
Sample preparation for proteomics and mass spectrometry [PDF File]
Jan 29, Fri E. Shonsey MS in Forensics [PDF File]
  Mid-term exam – take-home
Feb 2, Tu H. Kim Simplifying the proteome - techniques of protein purification; protein separation by electrophoresis and other 2D-methods [PDF File] [Questions]
Feb 5, Fri A. Smith IV Connecting proteomics into bioinformatics; MUDPIT and SEQUEST; false discovery rates in complex systems [PDF File]
Feb 9, Tu C. Crasto The bioinformatics of the proteome [PDF File]
Feb 12, Tu S. Barnes Mass spectrometry in qualitative and quantitative burrowing of the proteome [PDF File]
Feb 16, Fri J. Prasain Designing the proteomics and metabolomics experiment [PDF File] [Questions]
Feb 19, Fri J. Prasain Qualitative and quantitative analysis/method validation in metabolomics [PDF File] [Questions]
Feb 23, Tu S. Barnes Enzymology and mass spectrometry [PDF File]
Feb 26, Fri M. Renfrow Analysis of protein-protein interactions by affinity purification and mass spectrometry [PDF File 1] [PDF File 2] [PDF File 3] [Bring a copy of the supplemental info (not the whole thing, just the methods)]
Mar 2, Tu P. Prevelige Mass spectrometry as a tool for studying protein structure
Mar 5, Fri P. Prevelige Study of macromolecular structures - protein complexes
Mar 9, Tu M. Renfrow Applications of FT-ICR-MS
Mar 12, Fri J. Novak/
M. Renfrow
Mass spectrometry in glycomics research - application to IgA nephropathy [PDF File]
Mar 16, Tu C-C. Wang Tissue and body fluid proteomics and mass spectrometry
Mar 19, Fri J. Mobley/
D. Stella
Applications of MS to tissue imaging
Mar 23, Tu H. Kim Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases [PDF File]

Final report due