Protein mass spectrometry 2014
(BMG/PHR 744)
The science of Omics and mass spectrometry
Course will be held on Mondays, Wednesdays and Fridays 9 – 10:30 am in the McCallum Building, 4th floor conference room (401)
Schedule
| Date | Lecturer | Topic |
|---|---|---|
| Basics Course | ||
| Jan 6, Mon | S. Barnes | Analyzing biomolecules. The impact of –omics on biomedical research [PDF File] |
| Jan 8, Wed | H. Kim | Simplifying the proteome - techniques of protein purification [PDF File] |
| Jan 10, Fri | H. Kim | Protein separation by electrophoresis and other 2D-methods [PDF File] |
| Jan 13, Mon | M. Renfrow | The Mass Spectrum: What does it show you? MS1, MS2, MSn [PDF File] |
| Jan 15, Wed | M. Renfrow | Mass Spectrometry: Detecting and moving ions in the gas phase. Instrumentation, Mass Analyzers, Ionization [PDF File] |
| Jan 17, Fri | M. Renfrow | Biological Mass Spectrometry: Ionization, Calculating Mass, Charge [PDF File] |
| Jan 20, Mon | Martin Luther King Holiday | |
| Jan 22, Wed | M. Renfrow | Peptide/protein identification in MS, Search algorithms, false positives. |
| Jan 24, Fri | S. Barnes | Qualitative burrowing of the proteome – identifying PTMs [PDF File] |
| Jan 27, Mon | S. Barnes | Metabolomics – GC-MS, LC-MS and NMR [PDF File] |
| Jan 29, Wed | Snow day – University closed | |
| Jan 31, Fri | S. Barnes | MRMPath; MRMutation; MRMass Space [PDF File] |
| Feb 3, Mon | S. Barnes | Quantitative burrowing of the proteome – Labeling, label-free and absolute quantification [PDF File] |
| Feb 5, Wed | J. Prasain | Ion fragmentation of small molecules in mass
spectrometry [PDF File] Take home Exam |
| Applications Course | ||
| Feb 7, Fri | M. Renfrow | Analysis of protein-protein interactions by affinity purification and mass spectrometry |
| Feb 10, Mon | J. Prasain | Lipidomics and other small molecules by LC-MS [PDF File] |
| Feb 12, Wed | C. Crasto | Web tools and the proteome/metabolome; Expasy, KEGG, NCBI, others |
| Feb 14, Fri | M. Renfrow | Applications of FT-ICR-MS |
| Feb 17, Mon | J. Prasain | Quantitative analysis of small molecules in biological samples [PDF File] |
| Feb 19, Wed | J. Novak/ M. Renfrow |
Mass spectrometry in glycomics research - Application to IgA nephropathy |
| Feb 21, Fri | E. Shonsey |
Application of MS in Forensics [PDF File] |
| Feb 24, Mon | S. Barnes J Kabarowski |
Application of MS to tissue imaging [PDF File] |
| Feb 26, Wed | S. Barnes | Barriers to proteomics research – Federal viewpoint |
| Feb 28, Fri | Student presentations | |
| Mar 3, Mon | H. Kim | Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases |
| Mar 5, Wed | H. Kim S. Barnes |
Putting it all together – by-passing pyruvate kinase |
| Mar 7, Fri | P. Prevelige | Mass Spectrometry as a Tool for Studying Protein Structure [PDF File] |
| Mar 10, Mon | P. Prevelige | Study of macromolecular structures – protein complexes [PDF File] |
| Mar 12, Wed | S. Barnes | Isotopes in mass spectrometry |
| Mar 14, Fri | S. Barnes | Applying mass spectrometry to Free Radical Biology |
| Mar 21, Fri | Final report due |