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    High Resolution Imaging Facility at UAB
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    High Resolution Imaging Facility at UAB

Mission Statement

The High Resolution Imaging Facility (HRIF) provides state-of-the-art imaging resources and technical support to the UAB community. HRIF offers electron microscopy and light microscopy including confocal, live cell, multi-photon, widefield, super resolution, and image analysis. To effectively implement these technologies, we provide consultations, expert training, and support for all our systems. We are open to all UAB investigators as well as other investigators in the research community.


HRIF News & Events


  • 4 cutting-edge machines powering UAB discoveries

    Photo by ANDREA MABRY | University Relations

    Alexa Mattheyses, Ph.D. (right), director of the UAB High-Resolution Imaging Facility, and Robert Grabski, Ph.D., research associate in the High-Resolution Imaging Facility. They are pictured with the Zeiss Lightsheet 7 light sheet microscope, an advanced imaging device capable of fluorescent imaging of living cells and tissues.

    What it does: A light sheet fluorescent microscope, or LSFM, allows researchers to visualize the relationships between cells and proteins in intact tissues, organs and organisms at high volume and at a range of sizes and resolutions. Because of its image acquisition speed, the Zeiss Lightsheet 7 can perform data collection from large samples rapidly and efficiently, “allowing us to image far larger samples than we are currently capable of, including whole organisms and intact cleared tissues,” Mattheyses wrote in her grant abstract. Light sheet fluorescent microscopy also “substantially reduces photobleaching and photodamage,” she added. Samples do not need to be mounted on a slide or coverslip, “allowing more physiological imaging of model organisms and intact tissues,” Mattheyses said. “Finally, samples can be rotated relative to the imaging plane, allowing ideal orientation and collection of multiple views.”

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  • Coming by the end of 2023: New JEOL JEM-1400 Transmission Electron Microscope (TEM)!

    We are happy to announce that the TEM (Transmission Electron Microscope) Lab has relocated from the basement to the HRIF main location at the first floor of the Shelby building, and will be operational by the end of 2023. We will house a new JEOL TEM 120 kV with a AMT NanoSprint 43L Digital Camera, new Leica ultramicrotomes, and new accesory equipment. The flood recovery effort has been long and we appreciate everyone’s patience as we assume normal operations.

    • Compact footprint and simplified GUI with multitouch screen for optimal ease of use
    • Optimized for high contrast imaging for biological, low Z, and materials science applications
    • AMT-NanoSprint43L-MarkII: AMT-NanoSprint43L-MarkII Camera, 43MP, low mount position on electron column
    • +/- 70º tilt with support for dual axis tomography
    • Automated tomography from multiple points
    • Remote operation and data viewing for collaboration
    • LaB6 emitter standard
    • SerialEM software for montaging and tomography.

  • Zeiss Lightsheet 7 is here!

    We are very excited to announce that our Zeiss Lightsheet7 microscope, purchased with support of a NIH S10 grant, is fully operational! This microscope brings unique imaging capabilities previously unavailable to UAB research community, The Zeiss Lightsheet 7 Fluorescent Microscope (LSFM) is ideal for fast and gentle imaging of whole living model organisms (zebrafish, organoids, tadpole embryo, various tissues) as they develop over extended periods of time and imaging of large optically cleared specimens with subcellular resolution. Dedicated optics, sample chambers and holders allow adaption to the refractive index of your chosen clearing method.

  • HRIF presents Nikon AXR Confocal with NSPARC.

    We are very excited to host a seminar (August 29th, 2023, at 10AM, Shelby building room 105) and hands on demonstration (August, 30-31st, 2023, Shelby building, room 136a) of the newest and most advanced Nikon AXR confocal microscope with NSPARC. We sincerely invite all our patrons at UAB research community to attend the seminar and register for hands on demonstration.

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