This basic science and translational research program is focused on elucidating the cause-and-effect mechanisms of endotheliopathy that drive organ dysfunction and coagulation abnormalities in the setting of traumatic injury and hemorrhagic shock. In combination with clinical studies and animal models of injury and inflammation, we utilize custom-designed bioengineered cell culture systems to study the impact of resuscitation strategies on glycocalyx degradation and glycocalyx structural modifications that dictate mechanisms of vascular inflammation, endothelial hyperpermeability, aberrant coagulation and impaired vasoreactivity. Our research efforts are aimed at the identification of therapeutic strategies to protect, restore or modify the endothelial glycocalyx to mitigate endotheliopathy and improve clinical outcomes for trauma patients.