Protein mass spectrometry 2013
(BMG/PHR 744)
The science of Omics and mass spectrometry
Course will be held on Mondays, Wednesdays and Fridays 9 – 10:30 am in the McCallum Building, 4th floor conference room (401)
Schedule
| Date | Lecturer | Topic |
|---|---|---|
| Basics Course | ||
| Jan 7, Mon | S. Barnes | Analyzing biomolecules. The impact of –omics on biomedical research [PDF File] |
| Jan 9, Wed | H. Kim | Simplifying the proteome - techniques of protein purification [PDF File] |
| Jan 11, Fri | H. Kim | Protein separation by electrophoresis and other 2D-methods [PDF File] |
| Jan 14, Mon | M. Renfrow | The Mass Spectrum: What does it show you? MS1, MS2, MSn |
| Jan 16, Wed | M. Renfrow | Mass Spectrometry: Detecting and moving ions in the gas phase. Instrumentation, Mass Analyzers, Ionization |
| Jan 18, Fri | M. Renfrow | Biological Mass Spectrometry: Ionization, Calculating Mass, Charge | Jan 21, Mon | Martin Luther King Holiday |
| Jan 23, Wed | M. Renfrow | Peptide/protein identification my MS, Search algorithms, false positives. |
Jan 25, Fri | S. Barnes | Qualitative burrowing of the proteome – identifying PTMs [PDF File] |
| Jan 28, Mon | S. Barnes | Quantitative burrowing of the proteome – Labeling, label-free and absolute [PDF File] |
| Jan 30, Wed | C. Crasto | Web tools and the proteome/metabolome; Expasy, KEGG, NCBI, others [PDF File] |
| Feb 1, Fri | S. Barnes | MRMPath; MRMutation; MRMass Space | Feb 4, Mon | J. Prasain | Lipidomics and other small molecules by LC-MS [PDF File] | Feb 6, Wed | Exam (Possible lecture catch up and question session) |
| Applications Course | ||
| Feb 8, Fri | J. Prasain | Metabolomics – LC-MS, GC-MS and NMR [PDF File] |
| Feb 11, Mon | J. Prasain | Quantitative analysis/method validation in metabolomics [PDF File] |
| Feb 13, Wed | S. Barnes | Enzymology, metabolism and mass spectrometry [PDF File] |
| Feb 15, Wed | S. Barnes | Applications of MS to tissue imaging – the lens and the metabolome [PDF File] | Feb 18, Mon | M. Renfrow | Analysis of protein-protein interactions by affinity purification and mass spectrometry |
| Feb 20, Wed | M. Renfrow | Applications of FT-ICR-MS |
| Feb 22, Fri | J. Novak/ M. Renfrow |
Mass spectrometry in glycomics research - Application to IgA nephropathy | Feb 25, Mon | E. Shonsey | Application of MS in Forensics [PDF File] |
| Feb 27, Fri | Student presentations | |
| Mar 1, Fri | Student presentations | |
| Mar 4, Mon | P. Prevelige | Mass Spectrometry as a Tool for Studying Protein Structure |
| Mar 6, Wed | P. Prevelige | Study of macromolecular structures – protein complexes |
| Mar 8, Fri | H. Kim | Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases |
| Mar 11, Mon | H. Kim S. Barnes |
Putting it all together – by-passing pyruvate kinase |
| Mar 13, Wed | S. Barnes | Isotopes in mass spectrometry |
| Mar 15, Fri | S. Barnes | Applying mass spectrometry to Free Radical Biology |
| Mar 22, Fri | Final report due |