Protein mass spectrometry 2013
(BMG/PHR 744)

The science of Omics and mass spectrometry

Course will be held on Mondays, Wednesdays and Fridays 9 – 10:30 am in the McCallum Building, 4th floor conference room (401)

Schedule

Date Lecturer Topic
    Basics Course
Jan 7, Mon S. Barnes Analyzing biomolecules. The impact of –omics on biomedical research [PDF File]
Jan 9, Wed H. Kim Simplifying the proteome - techniques of protein purification [PDF File]
Jan 11, Fri H. Kim Protein separation by electrophoresis and other 2D-methods [PDF File]
Jan 14, Mon M. Renfrow The Mass Spectrum: What does it show you? MS1, MS2, MSn
Jan 16, Wed M. Renfrow Mass Spectrometry: Detecting and moving ions in the gas phase. Instrumentation, Mass Analyzers, Ionization
Jan 18, Fri M. Renfrow Biological Mass Spectrometry: Ionization, Calculating Mass, Charge
Jan 21, Mon   Martin Luther King Holiday
Jan 23, Wed M. Renfrow

Peptide/protein identification my MS, Search algorithms, false positives.

Jan 25, Fri S. Barnes Qualitative burrowing of the proteome – identifying PTMs [PDF File]
Jan 28, Mon S. Barnes Quantitative burrowing of the proteome – Labeling, label-free and absolute [PDF File]
Jan 30, Wed C. Crasto Web tools and the proteome/metabolome; Expasy, KEGG, NCBI, others [PDF File]
Feb 1, Fri S. Barnes MRMPath; MRMutation; MRMass Space
Feb 4, Mon J. Prasain Lipidomics and other small molecules by LC-MS [PDF File]
Feb 6, Wed   Exam (Possible lecture catch up and question session)
    Applications Course
Feb 8, Fri J. Prasain Metabolomics – LC-MS, GC-MS and NMR [PDF File]
Feb 11, Mon J. Prasain Quantitative analysis/method validation in metabolomics [PDF File]
Feb 13, Wed S. Barnes Enzymology, metabolism and mass spectrometry [PDF File]
Feb 15, Wed S. Barnes Applications of MS to tissue imaging – the lens and the metabolome [PDF File]
Feb 18, Mon M. Renfrow Analysis of protein-protein interactions by affinity purification and mass spectrometry
Feb 20, Wed M. Renfrow Applications of FT-ICR-MS
Feb 22, Fri J. Novak/
M. Renfrow
Mass spectrometry in glycomics research - Application to IgA nephropathy
Feb 25, Mon E. Shonsey Application of MS in Forensics [PDF File]
Feb 27, Fri   Student presentations
Mar 1, Fri   Student presentations
Mar 4, Mon P. Prevelige Mass Spectrometry as a Tool for Studying Protein Structure
Mar 6, Wed P. Prevelige Study of macromolecular structures – protein complexes
Mar 8, Fri H. Kim Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases
Mar 11, Mon H. Kim/S. Barnes Putting it all together – by-passing pyruvate kinase
Mar 13, Wed S. Barnes Isotopes in mass spectrometry
Mar 15, Fri S. Barnes Applying mass spectrometry to Free Radical Biology
Mar 22, Fri   Final report due