Central Alabama High-Field NMR Facility

Research Administration Centers and Cores Central Alabama High-Field NMR Facility

Central Alabama High-Field NMR Facility

Directory Category: Core

The Facility Staff will be happy to collect data as a service, or allow NMR-trained users to collect the data themselves. The staff provides advice and consultation to users in the following Research Capabilities useful In Structural Biology and Drug Design and Discovery Efforts:

  1. Quality Control ln Synthetic Chemistry and Medicinal Chemistry Drug Discovery/Design Projects by 1D/2D-NMR (1H, 13C, 19F, 31P, 195Pt. etc).
  2. Determination of the three-dimensional structures of proteins and their complexes (protein-protein, protein-nucleic acid, protein-carbohydrate).
  3. Protein complexes with low molecular weight compounds (including low-affinity compounds). The bound·structure of the compound can be used in Structure-based design of improved lead compounds and ultimately drugs.
  4. STD-NMR Screening (a binding assay) of single Compound or Compound Libraries to identify "hits'' recognized by a target protein. Group epitope mapping of ligands. STD-NMR/CORCEMA-ST analyses of bound-ligand conformations.
  5. Fragment-based discovery & design (lead optimization) of new drugs by: (a) SAR-by-NMR (a technique pioneered by the Abbott Laboratories, widely used in Pharmaceutical Industry), and the (b) ILOE technique
  6. Membrane proteins in solution (proteins in micelles and bicelles).
  7. NMR of cells, cell extracts, and Metabolomics-by-NMR