Hybridoma and Monoclonal Immunoreagent Core

Research Administration Centers and Cores Hybridoma and Monoclonal Immunoreagent Core

Hybridoma and Monoclonal Immunoreagent Core

Directory Category: Institutional Core

The core is currently in maintenance mode and no new antibodies can be produced at this time. If you have questions about cells or antibodies stored in the core, please contact Julie Decker.

The Hybridoma and Monoclonal Immunoreagent Core (formerly Epitope Recognition and Immunoreagent Core) provides expert development of new hybridoma lines as well as maintenance and antibody production from established hybridoma lines. It was established in 1982 as part of the UAB Arthritis Center and has been continuously funded through various NIAMS granting echanisms  since that time.  The HMIC performs all procedures related to monoclonal antibody (mAb) development including the design of immunizing antigens, mouse acquisiton and immunization, cell fusion and screening, subcloning and cryopreservation of hybridoma lines.  The core also provides large-scale mAb production from tissue culture supernatants. The price for production of 10-100 mgs of purified mAb ranges from $300 to $850. Due to its long-term association with the Arthritis Center, the HMIC maintains an extensive panel of hybridoma antibodies related to the study of mouse and human immune responses, as well as isotype matched KLH-specific negative control mAbs.