All investigators are strongly encouraged to contact Mitometabolism Core prior to sample submission to ensure proper handling and preparation.

Mitochondrial Bioanalytical Services

  • Mitochondrial Oxidative Phosphorylation Complex Activity Assays
  • Mitochondrial Protein Content Analysis (1D and/or 2D western blotting and Blue Native PAGE).
  • Mitochondrial Citrate Synthase Assay
  • Bioenergetics' Analysis of Tissues, Mitochondria, and Cells

Mitochondrial models and mtDAMPs

  • Mitochondrial nuclear exchange (MNX) models
  • mtDNA damage and haplotyping analysis
  • measurement of mtDAMPs

Autophagy and Mitophagy Assessments

  • Ratio of LC3 I and II proteins by western blot.
  • Autophagic flux assays

Targeted Proteomics and Metabolomics

  • F2-Isoprostane Measurements
  • Krebs cycle and glycolysis intermediates
  • GSH: GSSG ratio measurements

Education and Training in the measures of mitochondrial health and quality control

  • Consultations

Mitometabolism Core Fees

Targeted metabolomics and isoprostane assays require the use of the UAB Targeted Metabolomics and Proteomics Laboratory and will cost $200/sample (this includes sample preparation in NSC core at $140 / sample, as well as the UAB, Targeted Metabolomics and Proteomics Laboratory and will start at a rate of $60/sample). We will act as an intermediary for samples that need to be processed by mass spectrometry.

All other assays will start at a base cost of $200. A more thorough cost cannot be assessed until the experimental details have been discussed, i.e., sample matrix, sample number, and endpoint desired.