All investigators are strongly encouraged to contact Mitometabolism Core prior to sample submission to ensure proper handling and preparation.
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Mitochondrial Bioanalytical Services
- Mitochondrial Oxidative Phosphorylation Complex Activity Assays
- Mitochondrial Protein Content Analysis (1D and/or 2D western blotting and Blue Native PAGE).
- Mitochondrial Citrate Synthase Assay
- Bioenergetics' Analysis of Tissues, Mitochondria, and Cells
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Mitochondrial models and mtDAMPs
- Mitochondrial nuclear exchange (MNX) models
- mtDNA damage and haplotyping analysis
- measurement of mtDAMPs
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Autophagy and Mitophagy Assessments
- Ratio of LC3 I and II proteins by western blot.
- Autophagic flux assays
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Targeted Proteomics and Metabolomics
- F2-Isoprostane Measurements
- Krebs cycle and glycolysis intermediates
- GSH: GSSG ratio measurements
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Education and Training in the measures of mitochondrial health and quality control
- Consultations
Mitometabolism Core Fees
Targeted metabolomics and isoprostane assays require the use of the UAB Targeted Metabolomics and Proteomics Laboratory and will cost $200/sample (this includes sample preparation in NSC core at $140 / sample, as well as the UAB, Targeted Metabolomics and Proteomics Laboratory and will start at a rate of $60/sample). We will act as an intermediary for samples that need to be processed by mass spectrometry.
All other assays will start at a base cost of $200. A more thorough cost cannot be assessed until the experimental details have been discussed, i.e., sample matrix, sample number, and endpoint desired.