Cameron Sheeler, Ph.D., is an associate professor in the UAB Biotechnology program. He teaches BT650: Applications in Biotechnology I, BT651: Applications in Biotechnology II, and BT652: Applications in Biotechnology III.
In this series, Dr. Sheeler shows you important techniques, equations, and concepts that will help you in any scientific laboratory or classroom situation.
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C1V1 = C2V2
Learn how to use the C1V1 equation as it relates to preparing a cell concentration.
Known Concentration, Desired Volume
Learn how to use a known concentration to find the desired volume.
Serial Dilution
Dr. Sheeler expands on the C1V1 = C2V2 equation and you will learn how to do a serial dilution. He starts by using a Molar Stock of 1 Molar Magnesium Chloride (1M Mg Cl2) and will make a 25 milliMolar (mM) solution to start.
Calculate the Molarity of a 1 Percent Solution
Learn how to calculate the molarity of a 1.0% solution. Once again - Dr. Sheeler's example is Ethidium Bromide (EtBr), which he and his students use in his lab at UAB.
Molecular Formula to Calculate Molecular Weight
Learn how to use molecular formulas to calculate molecular weight. Dr. Sheeler's example is Ethidium Bromide (EtBr).
Make a Percent Solution
Learn how to make a 1.0% solution, a 0.9% solution AND a 2.0% solution. Dr. Sheeler says this procedure even works for gels.
Use a Working Stock Solution
Learn how to use working stock solutions in research applications.
Make a Working Stock Solution
Learn how to make a stock solution and then how to use a serial dilution to make a working stock solution for all of your experiments.
Using a DNA Ladder
Learn how to use a DNA ladder. Dr. Sheeler also gets into determining the size of DNA fragments and even touches on PCR fragments.