Stephen Barnes, Ph.D.

 

 barnes

Professor of Pharmacology and Toxicology

Professor of Biochemistry and Molecular Genetics

Professor of Environmental Health Sciences

Professor of Genetics

Professor of Vision Sciences

Director, UAB Center for Nutrient-Gene Interaction

Associate Director, Purdue-UAB Botanicals Centers for Age-Related Disease

Director, Targeted Metabolomics and Proteomics Laboratory (TMPL)

Senior Scientist, UAB Comprehensive Cancer Center

 

Education:

B.Sc. - University of Surrey, UK; Ph.D. - University of London, UK; postdoctoral training - Department of Chemical Pathology, St. Thomas' Hospital, London, UK and Royal Free Hospital Academic Department of Medicine, London, UK

 

Awards/Recognitions:

American Society for Mass Spectrometry 1994-present; American Society of Nutrition 2008; 1995-2006 Ad-hoc reviewer for NCI, NIH/ODS and NIDDK; 1997 Co-Chair, International Symposium on Phytoestrogen and research methods; 1999 Chairman, NIH/ODS Botanicals Center Study Section; 2001 Co-Chair, 4th Int’l Soy Symposium; 2003 Lifetime service award, 5thInt’l Soy Symposium; 2004 Organizing Committee, AICR International Research Conference on Food, Nutrition, and Cancer; Chair, AACR/WROTC Proteomics in Drug Discovery, 2005; Organizing Committee, Int’l Nutrigenomics Symposium, Auckland, New Zealand, 2006; Steering Committee, Oxidative Post-translational Modifications Symposium, Boston, 2006, 2008; Member, National Advisory Council, NCCAM, 2007-2010; Keynote speaker, 2007 FASEB meeting, non-antioxidant mechanisms for the action of flavonoids; Keynote speaker, Natural Health Products Research Society of Canada and the Canadian Herb, Spice and Natural Health Products Coalition, Saskatoon, Saskatchewan, Canada, 2007; Invited speaker, Biochemical Society Symposium on Bioanalysis in Oxidative Stress, Exeter, UK, 2008; Invited speaker, Translational Medicine in Cardiovascular and Pulmonary Diseases, Denver, CO, 2008; Invited speaker, American Institute for Cancer Research, Washington, 2008; Invited speaker, Society for Free Radical Biology & Medicine, Indianapolis, 2008; Organizing committee NCI Translational Science Meeting, Washington, DC, 2008; Blue Ribbon Panel on NCCAM Intramural Research Program, 2009; Plenary speaker, Soy Research Workshop, 2009; Member, NIH Council of Councils, 2009-2012; Invited speaker, 3rd Int’l Polyphenols Symposium, Harrogate, UK, 2009; NCCAM Think-Tank on the Future of Botanicals Research, 2010; Chair, Herbals and conventional drugs session, Consortium for the Globalization of Chinese Medicine, Hong Kong, China, 2010; Steering Committee and invited speaker, Utian Symposium on Translational Science, Menopause Society, 2010; Invited Speaker, Stars in Nutrition and Cancer, NCI, 2010; Invited speaker, Equol Session, 9Th International Soy Symposium, Washington, DC, 2010; invited speaker, PhenRIG meeting, FASEB, Washington, DC, 2011; Invited speaker, Office of Dietary Supplements in the NIH Division of Disease Prevention, 2011; panelist, NIH Human Proteome Workshop, 2011; Invited speaker, International Conference on Natural Bioactives for Health and Disease, London, Ontario, 2012; Fellow American Society of Nutrition, 2012.


Current Research Support:

NIH/NEI R21 EY020963-02   Lens crystallins: spatial location and properties in the ICR/f rat cataract model08/10-05/13 (PI)

Cystic Fibrosis Foundation (Eric Sorscher, Center Director) Post-translational modifications of CFTR07/11-06/13 (PI)

Department of Energy (L. Cseke, UAH, PI), Nutrient cycling for biomass: Interactive proteomic/transcriptomic networks for global carbon management processes within poplar-mycorrhizal interactions 09/13-08/14 (PI of subcontract)

NIH/NCI R01 CA082729-07 (Xiao-Ou Shu, PI), Cohort Study of Cancer-Inhibitory Dietary Factors in Men (Subcontract from Vanderbilt) 07/10-06/12 (PI of subcontract)

NIH NINDS P01 NS42803-03  (A. Messing, PI) Alexander Disease:  Cellular and Molecular Mechanisms 07/08-06/13 (Investigator on subcontract)

NIH/NCI R01 CA131653-02 (J. Lancaster, PI)Biological Chemistry of Reactive Nitrogen Species Signaling in Cancer Etiology 04/09- 03/14 (investigator)

NIH/NIAMS P30 AR50948-07 (PI: Craig Elmets), Skin Disease Research Center 09/09 – 08/14 (Proteomics Core Director)

NIH/NIDDK P30 DK079337-04 (Anupam Agarwal, PI)UAB-UCSD O'Brien Core Center for Acute Kidney Injury Research 07/08- 06/13 (Bioanalytical Core Director)

NIH/NIDDKP60 DK079626-03 (Tim Garvey, PI) UAB Diabetes Research and Training Center, Bioanalytical Redox Biology Core 03/08 - 02/13 (Bioanalytical Core Co-Director)

NIH/NIDDK P30 DK056336-08 (David Allison, PI)UAB Clinical Nutrition Research Unit 06/07 – 05/12 (Director, Pilot/Feasibility Program)


Selected Publications

2012

Higdon A, Landar A, Barnes S, Darley-Usmar VM.The Electrophile Responsive Proteome: Integrating Proteomics and Lipidomics with Cellular Function.Antioxid Redox Signal.2012 Feb 21.[Epub ahead of print]. PMID 22352679.

McClure M, DeLucas LJ, Wilson L, Ray M, Rowe S, Wu X, Dai Q, Hong JS, Sorscher EJ, Kappes JC, Barnes S. Palmitoylation and other post-translational modifications of CFTR with relevance to gating and processing.Protein Eng Des Sel. 2012 Jan;25(1):7-14. PMCID PMC3276306.

2011

Whitehouse-Tedd KM, Cave NJ, Ugarte CE, Waldron LA, Prasain JK, Arabshahi A, Barnes S, Thomas DG. Dietary isoflavone absorption, excretion and metabolism in captive cheetahs (Acinonyx jubatus).Journal of Zoo and Wildlife Medicine, 2011;42:658-670.PMID 22204061.

Li F-J, Kubagawa Y, McCollum M, Wilson L, Motohashi T, Bertoli LF, Barton JC, Barnes S, Davis RS, Kubagawa H. Enhanced levels of both membrane-bound and soluble forms of IgM Fc receptor in patients with chronic lymphocytic leukemia.Blood. 2011 Nov 3;118(18):4902-9PMCID PMC3208298.

 Li F, Olga V, Glinskii OV, Zhou J, Wilson LS, Barnes S, Anthony DC, Glinsky VV. Identification and Analysis of Signaling Networks Potentially Involved in Breast Cancer Metastasis to the Brain Using 2D-DIGE Proteomics and Ingenuity Pathway Analysis. PLoS One, 2011;6(7):e21977. Epub 2011 Jul 11. PMCID PMC3136937.

 Mirza S, Wilson L, Benjamin WH Jr, Novak J, Barnes S, Hollingshead SK, Briles DE. Serine protease PrtA from Streptococcus pneumoniae plays a role in the killing of S. pneumoniae by apolactoferrin. Infection and Immunity 2011 Jun;79(6):2440-502011.PMC3125832.

Rajbhandari R, Peng N, Moore R, Arabshahi A, Wyss JM, Barnes S, Prasain JK.Determination of Cranberry Phenolic Metabolites in Rats by Liquid Chromatography-Tandem Mass Spectrometry.J Agric Food Chem. 2011 Jun 22;59(12):6682-8. PMID: 21634376

Anderson JT, Zeng M, Li Q, Stapley R, Moore DR II, Chenna B, Fineberg N, Zmijewski J, Eltoum I-E, Siegal GP, Gaggar A, Barnes S, Velu SE, Thannickal VJ, Abraham E, Patel RP, Lancaster Jr JR, Chaplin DD, Dransfield MT, Deshane JS. Elevated levels of NO are localized to distal airways in asthma. Free Radical Biology and Medicine, 2011 Jun 1;50(11):1679-88.

Frölich MA, Arabshahi A, Katholi C, Prasain J, Barnes S. Hemodynamic characteristics of midazolam, propofol, and dexmedetomidine in healthy volunteers. Journal of Clinical Anaesthesiology, 2011 May;23(3):218-23

Floyd KA, Stella DR, Wang CC, Laurentz S, McCabe GP, Srivastava OP, Barnes S. Genistein and genistein-containing dietary supplements accelerate the early stages of cataractogenesis in the male ICR/f rat. Exp Eye Res. 2011 Feb;92(2):120-7.

Steinberg FM, Murray MJ, Lewis RD, Cramer MA, Amato P, Young RL, Barnes S, Konzelmann KL, Fischer JG, Ellis KJ, Shypailo RJ, Fraley JK, Smith EO, Wong WW. Clinical outcomes of a 2-year soy isoflavone supplementation in menopausal women.Am J Clin Nutr. 2011 Feb;93(2):356-67.

2010

Stella DR, Floyd KA, Grey AC, Renfrow MB, Schey KL, Barnes S. Tissue localization and solubilities of αA-crystallin and its numerous C-terminal truncation products in pre- and postcataractous ICR/f rat lenses. Investigative Ophthalmology and Vision Science, 51:5153-5161, 2010.  PMC3066599

Raska M,Takahashi K, Zachova K, Hall S, Moldoveanu Z, Elliott M, Wilson L, Brown R, Jancova D, Barnes S, Tomana M,  Smith PD, Mestecky J, Renfrow MB, Novak J. Glycosylation patterns of HIV-1 gp120 depend on the type of expressing cells and affect antibody recognition. Journal of Biological Chemistry, 285:20860-20869, 2010.PMC2898351

Pyle LC, Fulton JC, Sloane PA, Backer K, Mazur M, Prasain J, Barnes S, Clancy JP, Rowe SM. Activation of CFTR by the Flavonoid Quercetin: Potential Use as a Biomarker of ΔF508 CFTR Rescue. American Journal of Respiratory Cell and Molecular Biology, 43:607-616, 2010. PMC2970857.

Sun D, Zhuang X, Xiang X, Liu Y, Zhang S, Liu C, Barnes S, Grizzle W, Miller D, Zhang HG. A novel nanoparticle drug delivery system: the anti-inflammatory activity of curcumin is enhanced when encapsulated in exosomes. Mol Ther. 2010 Sep;18(9):1606-14.

Ma, T.C., Campana, A., Lange, P.S., Lee, H-H., Banerjee, K., Bryson, J.B., Mahishi, L., Giger, R.J., Estévez, A.G., Morris, S.M. Jr., Willis, D., Twiss, J., Filbin, M.T., Barnes, S., Ratan, R.R. A large-scale chemical screen for regulators of the arginase 1 promoter identifies the soy isoflavone, daidzein as a clinically approved, small molecule that can promote neuronal protection or regeneration via a cAMP-independent pathway. Journal of Neuroscience, 30:739-748, 2010.

Pyle LC, Fulton JC, Sloane PA, Backer K, Mazur M, Prasain J, Barnes S, Clancy JP, Rowe SM. Activation of CFTR by the Flavonoid Quercetin: Potential Use as a Biomarker of ΔF508 CFTR Rescue. American Journal of Respiratory Cell and Molecular Biology. In press.

Barnes S. Chemistry, Physiology and actions of soy isoflavones. Lymphatic Research and Biology, in press.

Prasain, JK, Arabshahi A, Moore DR, Greendale G, Wyss JM, Barnes S. Simultaneous determination of eleven phytoestrogens in human serum using a two minute liquid chromatography/tandem mass spectrometry method. Journal of Chromatography B, accepted for publication.

Barnes S, Kim H. Cautions and Research Needs identified at the Equol, Soy, and Menopause Research Leadership Conference. Journal of Nutrition, accepted for publication.

 

2009
Prasain, J.K., Peng, N., Moore, D.R. II, Arabshahi, A., Barnes, S., Wyss, J.M. Tissue distribution of puerarin and its conjugated metabolites in rat tissues by liquid chromatography tandem mass spectrometry. Phytomedicine, 16:65-71, 2009.

Prasain, J.K., Peng, N., Dai, Y., Moore, D. R., Arabshahi A., Wilson, L., Barnes, S., Wyss, J.M., Kim, H., Watts, R.L. Liquid Chromatography Tandem Mass Spectrometry Identification of Proanthocyanidins in Rat Plasma after Oral Administration of Grape Seed Extract. Phytomedicine, 16:233-43, 2009.

Peng, N., Prasain, J.K., Dai, Y., Moore, D.R., Arabshahi, A., Barnes, S., Carlson, S., Wyss, J.M. Chronic dietary Kudzu Isoflavones Improve Components of Metabolic Syndrome in Stroke-prone Spontaneously Hypertensive Rats.  Journal of Agricultural and Food Chemistry, 57: 7268-7273, 2009.

Wong, W.W., Lewis, R.D., Steinberg, F.M., Murray, M.J., Cramer, M.A., Amato, P., Young, R.L., Barnes, S., Ellis, K.J., Shypailo, R.J., Fraley, J.K., Konzelmann, K.L., Fischer, J.G., Smith, E.O. Soy isoflavone supplementation and bone mineral density in menopausal women: a two-year multi-center clinical trial. American Journal of Clinical Nutrition, 90:1433-1439, 2009.

Weaver, C.M., Martin, B.R., Jackson, G.S., McCabe G.P., Nolan, J.R., McCabe, L.D., Barnes, S., Reinwald, S., Boris, M.E., Peacock, P. Antiresorptive Effects of Phytoestrogen Supplements Compared to Estradiol or Risedronate in Postmenopausal Women Using 41Ca Methodology. Journal of Clinical Endocrinology and Metabolism 94:3798-3805, 2009.

Cox, K.B., Liu, J., Tian, L., Barnes, S., Yang, Q., Wood, P.A. Cardiac Hypertrophy in Mice with Long-Chain Acyl-CoA Dehydrogenase (LCAD) or Very Long-Chain Acyl-CoA Dehydrogenase (VLCAD) Deficiency. Laboratory Investigation, 2009;89:1348-54.

Smith, D.L., Jr., Nagy, T.R., Wilson, L.S., Dong, S., Barnes, S., Allison D.B. The Effect of Mannan Oligosaccharide Supplementation on Body Weight Gain and Fat Accrual in C57Bl/6J Mice. Obesity - in press.

Reinwald, S., Mayer, L.P., Hoyer, P.B., Turner, C.H., Barnes, S., Weaver, C.M. A Longitudinal Study of the Effect of Genistein on Bone in Two Different Murine Models of Diminished Estrogen-Producing Capacity. Journal of Osteoporosis, doi:10.4061/2010/145170 (on-line journal).

Legette, L.L., Martin, B.R., Shanazari, M., Lee, W-H., Helfreich, W.G., Qian, J., Waters, D.J., Barnes, S., Welch, J., Weaver, C.M. Supplemental dietary racemic equol has modest benefits to bone but has mild uterotropic activity in ovariectomized rats. Journal of Nutrition, 139:1908-1913, 2009.

2008
Kang DJ, Ridlon JM, Moore DR, Barnes S, Hylemon PB. Clostridium scindens baiCD and baiH genes encode stereo-specific 7 alpha/7 beta-hydroxy-3-oxo-Delta(4)-cholenoic acid oxidoreductases. Biochimica et Biophysica Acta. 1781(1-2):16-25, 2008.

Ashwell, M., Stone, E., Mathers, J., Barnes, S., Compston, J., Francis, R.M., Key, T., Cashman, K.D., Cooper, C., Khaw, K.T., Lanham-New, S., Macdonald, H., Prentice, A., Shearer, M. and Stephen, A. Nutrition and bone health projects funded by the UK Food Standards Agency: have they helped to inform public health policy? British Journal of Nutrition, 99: 198-205, 2008.

Shonsey, E.M., Eliuk, S.M., Johnson, M.S., Barnes, S., Falany, C.N, Darley-Usmar, V.M. and Renfrow, M.B. Inactivation of human liver bile acid CoA:amino acid N-acyltransferase by the electrophilic lipid, 4-hydroxynonenal. Journal of Lipid Research, 49, 282-294, 2008.

Mafia, K., Gupta, R., Kirk, M.C., Wilson, L., Srivastava, O.P., Barnes, S. UV-A-induced structural and functional changes in human lens deamidated αB-crystallin. Molecular Vision 14: 234-248, 2008.

Barnes, S., Birt, D.F., Cassileth, B.R. Cefalu, W.T., Chilton, F.H., Farnsworth, N.R., Raskin, I., van Breemen, R.B., Weaver, C.M. Technologies and experimental approaches in the NIH Botanical Research Centers. American Journal of Clinical Nutrition, 87: 476-480, 2008.

Weaver, C.M., Barnes, S., Wyss, J.M., Kim, H., Morré, D.M., Morré, D.J., Simon, J.E., Lila, M.A., Janle, E.M., Ferruzzi, M.G.. Botanicals for age-related diseases: from field to practice. American Journal of Clinical Nutrition, 87: 493-497, 2008.

Prasain J.K., Jones, K., Moore, D.R., Barnes, S., Leahy, M., Roderick, R., Juliana, M.M. and Grubbs, C.J. Effect of cranberry juice concentrate on chemically-induced urinary bladder cancers. Oncology Reports 19: 1565-1570, 2008.

Sarkar P, Sarkar S, Ramesh V, Kim H, Barnes S., Kulkarni A, Hall JC, Wilson BL, Thomas RL, Pellis NR, Ramesh GT. Proteomic Analysis of Mouse Hypothalamus under Simulated Microgravity. Neurochem Res. 33:2335-2341, 2008.

Barnes, S. Nutritional Genomics, Polyphenols, Diets, and Their Impact on Dietetics. Journal of the American Dietetic Association, 108:1888-1895, 2008.

Gadbury, G.L., Xiang, Q., Yang, L., Barnes, S., Page, G.P., Allison, D.B. Evaluating Statistical Methods Using Plasmode Data Sets in the Age of Massive Public Databases: An Illustration using False Discovery Rates. PLoS Genet 4(6): e1000098. doi:10.1371/journal.pgen.1000098.

Barnes, S., Shonsey, E.M., Eliuk, S.M., Stella, D., Mafia, K., Kim, H., Srivastava, O.P., Renfrow, M.B. High-resolution mass spectrometry and inactivation of protein targets during oxidant stress. Biochemical Society Transactions, 36:1037-1044, 2008.

Kim, H., Cope, M., Herring, R., Robinson, G., Wilson, L., Page, G.P., Barnes, S. 2D difference gel electrophoresis of pre-pubertal and pubertal rat mammary gland proteomesJournal of Proteome Research 7:4638-4630, 2008.

Prasain, J.K., Arabshahi, A., Moore, D.R. II, Barnes, S., Carroll, S. A liquid chromatography mass spectrometry assay for determination of PD168393, a specific and irreversible inhibitor of erbB membrane tyrosine kinases, in rat serum. Journal of Chromatography B, 876:219-224, 2008

Weaver, C.M., Barnes, S., Wyss, J.M., Kim, H., Morré, D.M., Morré, D.J., Simon, J.E., Lila, M.A., Janle, E.M., Ferruzzi, M.G. Research Highlights from the Purdue-UAB Botanicals Research Center for Age Related Diseases. Pharm Biol. 47: 768-773, 2009.

 
To contact Dr. Barnes:
1918 University Boulevard
MCLM 452
Birmingham, AL 35294-0005
Phone: (205) 934-7117
Fax: (205) 934-6944
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.