Advanced Graduate
Course BMG 744

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Jeevan Prasain, PhD

Jeevan Prasain, PhD

Assistant Professor
University of Alabama at Birmingham
Department of Pharmacology & Toxicology
456 McCallum Bldg.
1918 University Blvd.
Birmingham, AL 35294
Tel: (205) 996-2612
E-mail: Jprasain@uab.edu


Education:

M.Sc. (Chemistry) 1989, Tribhuvan University, Nepal
Ph.D. Toyama Medical & Pharmaceutical University, Japan

Research Experience

  1. Natural Product Chemistry
    Separation, purification and structure elucidation of bio-active natural products
    Structure elucidation of novel compounds by spectroscopic methods (NMR, MS, HPLC etc.)
    Chemical investigation on Taxus baccata, Alpinia blepharocalyx, Panax notoginseng, Euonymus hamiltonianus, Tribulus terrestis, Brazilian Propolis and Boenninghausenia albiflora

  2. Mass spectrometric analysis of natural products using ESI-MS tandem mass analysis

  3. Syntheses of macromolecules (carceplexes, hemicarceplexes, etc.)

  4. Cell-based assays like cell viability, nitric oxide inhibition, activation of cAMP, angiogenesis inhibition or activation, cell progression by flow cytometry

  5. Jeevan K. PrasainHigh throughput screening of chemical library

Publications

  • 2014
  • 2013
  • 2012
  • 2011
  • 2010
  • 2009
  • 2008
  • 2007
  • 2006
  • 2005
  • pre 2005
  1. McKnight K, Hoang HD, Prasain JK, Brown N, Vibbert J, Hollister KA, Moore R, Ragains JR, Reese J, Miller MA.
    Neurosensory perception of environmental cues modulates sperm motility critical for fertilization.
    Science. 2014 May 16;344(6185):754-7. [doi: 10.1126/science.1250598] [PMID: 24833393]

  2. Legette LL, Prasain J, King J, Arabshani A, Barnes S, Weaver CM.
    Pharmacokinetics of Equol, a Soy Isoflavone Metabolite, Changes with Form of Equol (Dietary vs. Intestinal Production) in Ovariectomized Rats.
    J Agric Food Chem. 2014 Jan 21 [PMID: 24446705]

  1. Han SM, El Oussini H, Scekic-Zahirovic J, Vibbert J, Cottee P, Prasain JK, Bellen HJ, Dupuis L, Miller MA.
    VAPB/ALS8 MSP Ligands Regulate Striated Muscle Energy Metabolism Critical for Adult Survival in Caenorhabditis elegans.
    PLoS Genet. 2013 Sep;9(9):e1003738. [PMID: 24039594] [doi: 10.1371/journal.pgen.1003738] Epub 2013 Sep 5.

  2. Whitehouse-Tedd K, Cave N, Ugarte C, Tucker L, Prasain JK, Arabshahi A, Barnes S, Hendriks W, Thomas D.
    Isoflavone metabolism in domestic cats (Felis catus): comparison of plasma metabolites detected after ingestion of two different dietary forms of genistein and daidzein.
    J Anim Sci. 2013 Mar;91(3):1295-306. [PMID: 23307849]

  3. Ulasova E, Perez J, Hill BG, Dubuisson JG, Bradley WE, Garber DW, Landar A, Barnes S, Prasain J, Parks DA, Dell’Italia LJ, Darley-Usmar VM.
    Quercetin prevents left ventricular hypertrophy in the Apo E knockout mouse.
    Redox Biol. 2013 Jul 16;1(1):381-6. [PMID: 24024175]

  4. Jeevan K. Prasain, Hieu D. Hoang, Johnathan W. Edmonds, Michael A. Miller
    Prostaglandin Extraction and Analysis in Caenorhabditis elegans
    Advances in Bioinformatics, J. Vis. Exp. (76), e50447 (2013) [doi:10.3791/50447]

  5. Hieu D. Hoang, Jeevan K. Prasain, Dixon Dorand, Michael A. Miller
    A Heterogeneous Mixture of F-Series Prostaglandins Promotes Sperm Guidance in the Caenorhabditis elegans Reproductive Tract
    PLoS Genet 9(1): e1003271 [doi:10.1371/journal.pgen.1003271]

  6. Jeevan K. Prasain, Alireza Arabshahi, Pam R. Taub, Scott Sweeney, Ray Moore, J. Daniel Sharer, Stephen Barnes.
    Simultaneous quantification of F2-isoprostanes and prostaglandins in human urine by liquid chromatography tandem-mass spectrometry
    Journal of Chromatography B. Volumes 913–914, 15 January 2013, Pages 161–168 doi: 10.1016/j.jchromb.2012.12.009.
  1. Prasain JK, Peng N, Rajbhandari R, Wyss JM.
    The Chinese Pueraria root extract (Pueraria lobata) ameliorates impaired glucose and lipid metabolism in obese mice.
    Phytomedicine. 2012 Nov 1. pii: S0944-7113(12)00353-4. doi: 10.1016/j.phymed.2012.09.017. [PMID: 23123226] [Epub ahead of print]

  2. He L, Kim T, Long Q, Liu J, Wang P, Zhou Y, Ding Y, Prasain J, Wood PA, Yang Q.
    Carnitine Palmitoyltransferase-1b (CPT1b) Deficiency Aggravates Pressure-Overload-Induced Cardiac Hypertrophy due to Lipotoxicity.
    Circulation. 2012 Aug 29. [Epub ahead of print] [PMID: 22932257]

  3. Edited by: Jeevan K. Prasain
    Tandem Mass Spectrometry - Applications and Principles
    ISBN 978-953-51-0141-3, Hard cover, 794 pages
    Publisher: InTech, Free Online Book
    Publication date: February 2012

  1. Whitehouse-Tedd KM, Cave NJ, Ugarte CE, Waldron LA, Prasain JK, Arabshahi A, Barnes S, Thomas DG.
    Dietary isoflavone absorption, excretion, and metabolism in captive cheetahs (Acinonyx jubatus).
    J Zoo Wildl Med. 2011 Dec;42(4):658-70. [PMID: 22204061] [doi:10.1638/2011-0060.1]

  2. Frölich MA, Arabshahi A, Katholi C, Prasain J, Barnes S.
    Hemodynamic characteristics of midazolam, propofol, and dexmedetomidine in healthy volunteers. J Clin Anesth. 2011 May;23(3):218-23. [PMID: 21570617]

  3. Edmonds JW, McKinney SL, Prasain JK, Miller MA.
    The gap junctional protein INX-14 functions in oocyte precursors to promote C. elegans sperm guidance.
    Dev Biol. 2011 Aug 25. [Epub ahead of print] [PMID: 21889935]

  4. Barnes S, Prasain J, D'Alessandro T, Arabshahi A, Botting N, Lila MA, Jackson G, Janle EM, Weaver CM.
    The metabolism and analysis of isoflavones and other dietary polyphenols in foods and biological systems.
    Food Funct. 2011 May 25;2(5):235-44. [PMID: 21779561]

  5. Rajbhandari, R, Peng, N, Moore, DR, Arabshahi, A, Wyss, JM, Barnes S, Jeevan K. Prasain JK.
    Determination of cranberry phenolic metabolites in rats by liquid chromatography-tandem mass spectrometry.
    J Agric Food Chem, 2011, 59(12):6682-8. [PMID: 21634376]

  6. Byer SJ, Eckert JM, Brossier NM, Clodfelder-Miller BJ, Turk AN, Carroll AJ, Kappes JC, Zinn KR, Prasain JK, Carroll SL.
    Tamoxifen inhibits malignant peripheral nerve sheath tumor growth in an estrogen receptor-independent manner.
    Neuro Oncol. 2011 Jan;13(1):28-41. [PMID:21075781]

  1. Edmonds JW, Prasain JK, Dorand D, Yang Y, Hoang HD, Vibbert J, Kubagawa HM, Miller MA.
    Insulin/FOXO Signaling Regulates Ovarian Prostaglandins Critical for Reproduction.
    Dev Cell. 2010 Dec 14;19(6):858-71. [PMID: 21145501]

  2. Prasain JK, Arabshahi A, Moore DR 2nd, Greendale GA, Wyss JM, Barnes S.
    Simultaneous determination of 11 phytoestrogens in human serum using a 2 min liquid chromatography/tandem mass spectrometry method.
    J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Apr 15;878(13-14):994-1002. Epub 2010 Mar 6. [PMID: 20346741]

  3. Prasain JK, Carlson SH, Wyss JM.
    Flavonoids and age-related disease: Risk, benefits and critical windows.
    Maturitas. 2010 Feb 22. [Epub ahead of print] [PMID: 20181448]
  1. Pyle LC, Fulton JC, Sloane PA, Backer K, Mazur M, Prasain J, Barnes S, Clancy JP, Rowe SM.
    Activation of CFTR by the Flavonoid Quercetin: Potential Use as a Biomarker of ΔF508 CFTR Rescue
    Am J Respir Cell Mol Biol. 2009 Dec 30. [PMID: 20042712]

  2. Peng N, Prasain JK, Dai Y, Moore R, Arabshahi A, Barnes S, Carlson S, Wyss JM.
    Chronic dietary kudzu isoflavones improve components of metabolic syndrome in stroke-prone spontaneously hypertensive rats.
    J Agric Food Chem. 26;57(16):7268-73, 2009.. [PMID: 19938872]

  3. Prasain JK, Peng N, Dai Y, Moore R, Arabshahi A, Wilson L, Barnes S, Michael Wyss J, Kim H, Watts RL.
    Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract.
    Phytomedicine, 16(2-3):233-43, 2009. [PMID: 19095430]

  4. Prasain JK, Peng N, Moore R, Arabshahi A, Barnes S, Wyss JM.
    Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography-tandem mass spectrometry.
    Phytomedicine, 16(1); 65-71, 2009. [PMID: 19027277]

  1. Prasain JK, Arabshahi A, Moore R, Barnes S, Carroll SL.
    A liquid chromatography mass spectrometry assay for determination of PD168393, a specific and irreversible inhibitor of erbB membrane tyrosine kinases, in rat serum.
    Journal of Chromatogr B. 876(2): 219-224, 2008

  2. Prasain J.K., Jones, K., Moore, D.R., Barnes, S., Leahy, M., Roderick, R., Juliana, M.M. and Grubbs, C.J.
    Effect of cranberry juice concentrate on chemically-induced urinary bladder cancers.
    Oncology Reports 19: 1565-1570, 2008.

  3. Carlson, S., Peng, N., Prasain, J.K. and Wyss, J.M.
    The effects of botanical dietary supplements on cardiovascular, cognitive and metabolic function in males and females.
    Gender Medicine, 5: S76-S90, 2008.

  4. Peng, N., Prasain, J.K., Dai, Y., Carlson, S.H. and Wyss, J.M.
    Dietary kudzu root extract supplementation improves glucose tolerance and plasma lipid profiles in Spontaneously Hypertensive Rats (SHR) fed a basal, but not high, NaCl diet.
    Abstracts of Experimental Biology, 22:948.9, 2008.

  5. Carlson, S.H., Peng, N., Prasain, J.K., Dai, Y and Wyss, J.M.
    Kudzu root extract improves glycemic control in non-glucose-impaired mice.
    Abstracts of Experimental Biology, 22:949.12, 2008.

  1. Prasain, J.K., Barnes, S.
    Metabolism and bioavailability of flavonoids in chemoprevention: current analytical strategies and future prospectus.
    Molecular Pharmaceutics. 4 (6), 846-864, 2007.

  2. Prasain J, Peng N, Acosta E, Moore R, Arabshahi A, Meezan E, Barnes S, Wyss, JM.
    Pharmacokinetic study of puerarin in rat serum by liquid chromatography tandem mass spectrometry.
    Biomedical Chromatography.

    Biomed Chromatogr. 21(4):410-4, 2007.

  3. Prasain, J.K., Reppert, A., Jones, K., Moore, D.R., II, Barnes, S., and Lila, M.A.
    Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry.
    Phytochemical Analysis 18:50-59, 2007.

  1. Barnes, S., Prasain, J.K., Wang, C-C., Moore, D.R., II.
    Applications of LC-MS in the study of the uptake, distribution, metabolism and excretion of bioactive polyphenols from dietary supplements.
    Life Sciences, 27;78(18):2054-9, 2006.

  2. Prasain JK, Xu J, Kirk M, Smith Johnson M, Sfakianos J, Barnes S.
    Differential biliary excretion of genistein metabolites following intraduodenal and intravenous infusion of genistin in female rats.
    J Nutr. 136:2975-9, 2006.

  1. D'Alessandro, TL., Boersma-Maland, BJ., Peterson, TG., Sfakianos, J., Prasain, JK., Patel RP, Darley-Usmar VM, Botting NP, Barnes S.
    Metabolism of phytoestrogen conjugates.
    Methods Enzymol. 400, 316-42, 2005.

  2. Meezan, E., Meezan, E., Jones, K., Moore, R., Barnes, S., Prasain, J.
    Contrasting effects of puerarin and daidzin on glucose homeostasis in mice.
    Journal of Agriculture and Food Chemistry, 53, 8760-8767, 2005.

  3. Peng, N., Clark, J., Prasain, J., White C., Kim, H., Wyss, J.
    Antihypertensive and cognitive effects of grape polyphenols in estrogen-depleted, female, spontaneously hypertensive rats.
    Am J Physiol Regul Integr Comp Physiol. 289: R771-R775, 2005.

  4. Barnes, S., Prasain, J.K.
    Current Progress in the use of Traditional Medicines and Nutraceuticals.
    Current Opinion in Plant Biology 8: 324-328, 2005.

  5. Prasain, J.K., Moore, R., Hurst, J., Barnes, S., van Kuijk, FJGM.
    Electrospray Tandem Mass Spectrometric Analysis of Carotenoids and their Oxidation Products.
    Journal of Mass Spectrometry 40: 916-923, 2005.

  1. Kim, H., Hall, P., Smith, M., Kirk, M., Prasain, J.K., Barnes, S., Grubbs, C.
    Chemoprevention by grape seed extract and genistein in carcinogen-induced mammary cancer in rats is diet-dependent.
    Journal of Nutrition, J Nutr. 134: 3445S-3452S, 2004.

  2. Prasain J.K., Wang C.-C., Barnes S.
    Mass spectrometric methods for the determination of flavonoids in biological samples.
    Free Radical Biology & Medicine, 37: 1324-1350, 2004.

  3. Prasain J.K., Jones K., Brissie, N., Moore D.R. II, Wyss J.M., Barnes S.
    Identification of puerarin and its metabolites in rats by liquid chromatography-tandem mass spectrometry.
    Journal of Agricultural Food Chemistry, 52: 3708-3712, 2004.

  4. Boersma BJ, D'Alessandro T, Benton MR, Kirk M, Wilson LS, Prasain J, Botting NP, Barnes S, Darley-Usmar VM, Patel RP.
    Neutrophil myeloperoxidase chlorinates and nitrates soy isoflavones and enhances their antioxidant properties.
    Free Radical Biology & Medicine, 35:1417-30, 2003.

  5. D'Alessandro T. Prasain J. Benton MR. Botting N. Moore R. Darley-Usmar V. Patel R. Barnes S.
    Polyphenols, inflammatory response, and cancer prevention: chlorination of isoflavones by human neutrophils.
    Journal of Nutrition. 133(11 Suppl 1):3773S-3777S, 2003.

  6. Prasain, J.K., Jones, K., Kirk, M., Wilson, L., Smith-Johnson, M., Weaver, C.M., Barnes, S.
    Profiling and quantification of isoflavonoids in kudzu dietary supplements by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry.
    J Agric Food Chem, 51:4213-4218, 2003.

  7. Prasain JK. Patel R. Kirk M. Wilson L. Botting N. Darley-Usmar VM. Barnes S.
    Mass spectrometric methods for the analysis of chlorinated and nitrated isoflavonoids: a novel class of biological metabolites.
    Journal of Mass Spectrometry. 38:764-771, 2003.

  8. Belenky M. Prasain J. Kim H. Barnes S.
    DING, a genistein target in human breast cancer: a protein without a gene.
    Journal of Nutrition 133(7 Suppl):2497S-2501S, 2003.

  9. Kadota S. Tezuka Y. Prasain JK. Ali MS. Banskota AH.
    Novel diarylheptanoids of Alpinia blepharocalyx.
    Current Topics in Medicinal Chemistry. 3:203-225, 2003.

  10. Wang C.-C., Prasain J.K., Barnes S.
    Review of the methods used in the determination of phytoestrogens.
    J. Chromatogr. B 777: 3-28, 2002.

  11. Prasain JK. Ueki M. Stefanowicz P. Osada H.
    Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry.
    Journal of Mass Spectrometry 37:283-291, 2002.

  12. Prasain JK. Stefanowicz P. Kiyota T. Habeichi F. Konishi Y.
    Taxines from the needles of Taxus wallichiana.
    Phytochemistry. 58:1167-70, 2001

  13. Stefanowicz P. Prasain JK. Yeboah KF. Konishi Y.
    Detection and partial structure elucidation of basic taxoids from Taxus wallichiana by electrospray ionization tandem mass spectrometry.
    Analytical Chemistry. 73:3583-3589, 2001

  14. K. Sekiya, Y. Tezuka, K. Tanaka, J. K. Prasain, T. Namba, K. Katayama, T. Koizumi, M. Maeda, T. Kondo, S. Kadota.
    Distribution, metabolism and excretion of butylidenephthalide of Ligustici chuanxiong rhizoma in hairless mouse after dermal application.
    J. Ethnopharmacol., 2000,71, 401-409.

  15. J. K. Prasain, J-X. Li, Y. Tezuka, K. Tanaka, P. Basnet, H. Dong, T. Namba, S. Kadota.
    A New Antiplatelet Diarylheptanoid from Alpinia blepharocalyx.
    Planta Med., 1999, 65, 196.

  16. J. K. Prasain, J-X. Li, Y. Tezuka, K. Tanaka, P. Basnet, H. Dong, T. Namba, S. Kadota.
    Calyxin H, Epicalyxin H, and Blepharocalyxins A and B, Novel Diarylheptanoids from the Seeds of Alpinia blepharocalyx.
    J. Nat. Prod., 1998, 61, 212-216.

  17. J. K. Prasain, Y. Tezuka, J-X. Li, K. Tanaka, P. Basnet, H. Dong, T. Namba, S. Kadota.
    Novel Diarylheptanoids from the Seeds of Alpinia blepharocalyx-Revised Structure of Calyxin A.
    J. Chem. Res., (M), 1998, 265-279.

  18. P. Peungvicha, S. S. Thirawrapan, R. Temsiririrkkul, H. Watanabe, J. K. Prasain, S. Kadota.
    4-Hydroxybenzoic acid: A Hypoglycemic Constituent of Aqueous Extract of Pandanus odorus root.
    J. Ethnopharm., 1998, 60, 27-32.

  19. J. K. Prasain, Y. Tezuka, K. Hase, P. Basnet, H. Dong, T. Namba, S. Kadota.
    Inhibitory Effect of Diarylheptanoids on Nitric Oxide Production in Activated Murine macrophages.
    Biol. Pharm. Bull., 1998, 21(4), 371-4.

  20. J. K Prasain
    "Bio-active Novel Diarylheptanoids from Alpinia blepharocalyx K.Schum" - Ph.D Thesis,
    Toyama Medical & Pharmaceutical University, 1998, Japan

  21. J-X. Li, Q. Shi, Q. B. Xiong, J. K. Prasain, Y. Tezuka, T. Hareyama, Z. T. Wang, K. Tanaka, T. Namba, S. Kadota.
    Tribulusamide A and B, new hepatoprotective lignanamides from the fruits of Tribulus terrestris: indications of cytoprotective activity in murine hepatocyte culture.
    Planta Medica., 1998, 64, 628-631.

  22. A. H. Banskota, Y. Tezuka., J. K. Prasain, K. Matsushige, I. Saiki., S. Kadota.
    Chemical Constituents of Brazilian Propolis and their Cytotoxic Activities.
    J. Nat. Prod., 1998, 61, 896-900.

  23. H. Li, J.-X. Li, J. K. Prasain, Y. Tezuka, T. Namba, T. Miyahara, S. Tonam, H. Seto, T. Tada, S. Kadota.
    Antiosteoporotic Activity of the Stem of Sambucus sieboldiana.
    Biol. Pharm. Bull., 1998, 21, 594-598.

  24. J. K. Prasain, Y. Tezuka, J.-X. Li, K. Tanaka, P. Basnet, H. Dong, T. Namba, S. Kadota.
    Six Novel Diarylheptanoids Bearing Chalcone or Flavanone Moiety from the Seeds of Alpinia blepharocalyx.
    Tetrahedron, 1997, 53, 7833-7842.

  25. 26. S. Kadota, J. K. Prasain, J.-X. Li, P. Basnet, H. Dong, T. Tani, T. Namba.
    Blepharocalyxins A and B, Novel Diarylheptanoids from Alpinia blepharocalyx, and their Inhibitory Effect on NO Formation in Murine Macrophages.
    Tetrahedron Lett., 1996, 37, 7283-7286.

  26. J. K. Prasain, S. Kadota, P. Basnet, K. Hase, T. Namba.
    Hepatoprotective Effects of Panax notoginseng: Gensenosides-Re and-Rg1 as its Active Constituents in D-galactosamine/lipopolysaccharide-induced Liver Injury.
    Phytomedicine, 1996, 2, 297-303.

  27. J. K. Prasain, P. Basnet, K. Hase, S. Kadota, T. Namba.
    Novel Diarylheptanoids from the Seeds of Alpinia blepharocalyx and their Inhibitory Activity on Nitric Oxide Formation.
    J. Trad. Med., 1996, 13, 448-449.

  28. S. Kadota, H. Dong, P. Basnet, J. K. Prasin, G-J. Xu, T. Namba.
    Three Novel Diarylheptanoids, Calyxin A, Calyxin B, and 3-Epicalyxin B from a Chinese Crude drug "Yunnan Cao Kou"
    (Alpinia blepharocalyx K. Schum.)
    Chem. Pharm. Bull., 1994, 42, 2647.