On this page are a few examples of special processing & embedding done in the CPL.Sainte-Marie processing for immunohistochemistryFixation in cold ethanol and hand processing for IHC (Sainte-Marie G. A paraffin embedding technique for studies employing immunofluorescence. J Histochem Cytochem 10:250-256, 1962)Colitis models for inflammatory bowel disease researchFor these projects, the colon is opened, cut into proximal, middle, and distal segments, and the proximal end of each segment is marked with India ink. The segments are fixed in cassettes using one biopsy pad to prevent curling. After fixation, each segment is trimmed into two strips, which are then embedded with proximal, middle, and distal segments placed side by side in the block. This allows the location and distribution of affected areas to be determined and provides good perpendicular sectioning for evaluation of colitis severity.Experimental autoimmune encephalomyelitis (EAE)Here the spinal column is fixed intact, demineralized, and trimmed into 2 mm thick segments. Segments are positioned in casettes in 3 x 3 arrays between biopsy pads for processing, and are embedded as positioned at trimming. This allows the distribution of lesions over the length of the spinal cord to be determined.
All National Science Foundation (NSF) systems, including the NSF website, Research.gov and FastLane, will be unavailable from 5:00 PM on Saturday, September 17, until 4:00 PM on Sunday, September 18. This downtime is scheduled to coincide with repairs to the Foundation's electrical systems. Affected deadlines that fall on Monday, September 19, are extended to Tuesday, September 20, submitter's local time. See the attached NSF downtime notice for more information on affected solicitations.
Effective September 8, 2016, Signing Officials have the ability to electronically request the withdrawal of an application through eRA Commons. A Signing Official has two business days after successfully submitting an error-free application to reject the application. If more than two business days have elapsed, the Signing Official must contact the Division of Receipt and Referral (DRR) to request an application be withdrawn. Contacting DRR directly may now be done electronically. Requesting the withdrawal of an application will remain available while the application has not undergone review and the summary statement posted.
For more details on the features listed above, please review the original announcement. For more information concerning Prior Approval for Withdrawals, please see Guide Notice NOT-OD-16-143 and the Prior Approval section in the eRA Commons online help.
The National Heart, Lung, and Blood Institute (NHLBI) have announced an important clarification impacting years of support for both new and renewal K24 funding. The maximum years of support is capped at 10 years. Review the full notice for more details.
The NIH Extramural Nexus Updates for August 2016 are now available. Some highlighted stories include: