Special Stains

Special Stains

Pathology Histopathology Special Stains

Special Stains

Special stains (histochemical methods) are used to help visualize and/or identify structures and substances in sections. CPL can provide most standard special stains. Listed below are a few examples.

Acid fast stains (various methods) for mycobacteria [Mycobacterium avium, lung, mouse]
Congo red for amyloid [Islet amyloid, macaque (polaroscopy)]
Gram stains (various methods) for bacteria [Corynebacterium bovis, skin, nude mouse]
Grocott’s methenamine silver (GMS) for fungi [Pneumocystis carinii, lung, Rag2-/- mouse]
Jones methenamine silver for basement membrane and mesangium [Glomerulonephritis, mouse]
Luxol fast blue-PAS for demyelination [Demyelinating myelitis, mouse]
Masson’s trichrome for collagen & muscle [Renal arteritis, mouse]
Periodic acid-Schiff (PAS) [Hypersensitivity bronchitis, mouse (PAS-hematoxylin)]
Phosphotungstic acid-hematoxylin (PTAH) for fibrin [Glomerular thrombosis, mouse]
Prussian blue for ferric iron [Hemosiderin, spleen, mouse]
Sudan black for lipids & lipochrome pigments [Lipochrome pigment, heart, ruffed lemur]
Verhoeff's method for elastin [Renal arteritis, mouse]
Warthin-Starry method for bacteria [Helicobacter hepaticus, liver, mouse]

Additional News and Announcements

  • New UAB Institutional Core Facility Program Launched

    The UAB Office of the Vice President for Research and Economic Development (OVPRED) announces the launch of the UAB Institutional Core Facility Program (ICFP), created to promote the development and operation of outstanding Core Facilities that can serve the needs of UAB investigators.
    Posted by Office of the Vice President for Research and Economic Development | 10/02/15
  • White Paper Solicitation for Major New Alabama Research Initiatives

    ​Alabama EPSCoR (ALEPSCoR) would like to solicit White Papers for major new research initiatives in targeted areas where Alabama can achieve national competitiveness and leadership. The goal of these targeted research initiatives will be to create the necessary State-wide research and education infrastructure to foster ground-breaking technology innovations resulting in substantial federal grant funding and intellectual property development that will create jobs for the State. The desired outcome of this white paper solicitation is to select one or two major new research initiatives for future investment and large scale multi-institutional proposal submissions.
    Posted by Office of the Vice President for Research and Economic Development | 09/16/15
  • Dr. Rakesh Patel, Ph.D. announced as new Director for the Center for Free Radical Biology

    Dr. Selwyn Vickers, M.D., F.A.C.S, Senior Vice President for Medicine and Dean, School of Medicine, and Dr. Richard Marchase, Ph.D., Vice President for Research and Economic Development, are pleased to announce that Rakesh Patel, Ph.D., will be the new director of the Center for Free Radical Biology (CFRB) from October 1, 2015, when Dr. Victor Darley-Usmar, Ph.D. steps down after serving as Director since 2006.
    Posted by Office of the Vice President for Research and Economic Development | 09/10/15
  • Change in State Law Regarding Age of Consent

    On May 7, 2015, Governor Bentley signed into law Act 2015-167, which lowered the age of consent to 18 years old (previously 19 years) for IRB-approved research conducted by an accredited college or university. This means that 18 year olds may now consent for themselves to participate in research conducted at UAB under IRB approval. The IRB has updated most of its forms with this information and will continue to revise policies and procedures to recognize this change in the law. The revised forms must be used for all submissions received in the OIRB after September 1, 2015.
    Posted by Office of the Institutional Review Board for Human Use | 06/26/15
  • IRB Form Changes - June 2015

    UAB IRB forms have been revised as of June, 2015. New versions of these forms will be required for all submissions received after September 1, 2015. These include: Human Subjects Protocol, Investigator's Progress Report, Sample Consent Form, Checklists for new submissions for both full and expedited reviews, Checklists for renewal submissions for both full and expedited reviews.
    Posted by Office of the Institutional Review Board for Human Use | 06/26/15
  • Confidential Disclosure Agreement Templates Updated

    UAB’s template confidential disclosure agreements have been updated and are available at the OSP Confidentiality Disclosure Agreement (CDA) webpage. The following confidential disclosure agreement (CDA) templates are available:

    Mutual Confidential Disclosure Agreement (Company and UAB disclosing confidential information);

    Unilateral Confidential Disclosure Agreement (Company Disclosing to UAB); and

    Unilateral Confidential Disclosure Agreement (UAB Disclosing to Company)

    Please use, and encourage the contracting party to use, the appropriate UAB template CDA. If the contracting party can agree to the terms of one of UAB’s template CDAs, UAB will sign the agreement without further routing and review, resulting in a more expeditious process.

    Please forward any questions regarding the CDA process, or UAB’s template CDAs to askosp@uab.edu.

    Posted by Office of Sponsored Programs | 06/22/15
  • Policy Update: Definition of a Principal Investigator

    Effective May 18, 2015 UAB’s Definition of a Principal Investigator policy is modified with regard to UAB employees in the 02 (Full-time Temporary) employment status or other UAB employment categories. See the full policy at the link or via the UAB Policies & Procedures Library.

    Case-by-case inquiries should be addressed to askosp@uab.edu.
    Posted by Office of Sponsored Programs | 06/01/15
  • Expired Form FDA 1572 continued use approved

    ​On April 30, 2015 the Form FDA 1572 on the FDA website at http://www.fda.gov/AboutFDA/ReportsManualsForms/Forms/ListFormsAlphabetically/ is expired.

    The FDA is in the process of updating and posting a new Form FDA 1572. Even though it is expired, the FDA approved continued use of the current form.

    We will monitor the FDA website and will post notice of the new form's release, when available.

    Posted by Office of the Institutional Review Board for Human Use | 05/06/15
  • Additional OSP Dropbox Enhancements

    The Office of Sponsored Programs is pleased to announce additional enhancements to the OSP dropbox for electronic SF424 grant applications to grants.gov. The link to access the OSP dropbox is OSP Drop Box for Electronic Submissions to Grants.gov . The revised OSP dropbox user guide can be found at OSP Dropbox Quick Submission Guide. New enhancements to the dropbox include:

    • The attribute page has been modified to prompt the user to create a record and then upload the files. When the proposal record is created the attribute page is refreshed with the comment “Submit Successful*” with a footnote to continue to upload files (“*Please continue on to the Upload Files section and attach your proposal documents”).

    • Once the proposal record is created, the Upload file tab is now used to upload the proposal documents.

    • The upload file history can now be viewed using the “File History” section.  This allows users to verify that the upload was successful.

    If you have any questions regarding the new OSP dropbox functionality, please contact your OSP federal officer or askosp@uab.edu

    Posted by Office of Sponsored Programs | 04/21/15
  • Subrecipients Under Uniform Guidance

    ​Per the new Uniform Guidance regulations, which became effective December 26, 2014, UAB “…must make case-by-case determinations whether each agreement it makes for disbursement of federal program funds casts the party receiving the funds in the role of a subrecipient or a contractor.” See Changes in Financial Management of Sponsored Projects Presentation 2 (12/14/2014) for additional information.
    Two documents have been developed to help determine whether a subaward (subcontract) or a fee-for-service/procurement contract is more appropriate. The Statement of Work (SOW) Template assures that there is a complete description of the work to be performed.  The Third Party Agreement Determination Form, in combination with the SOW, can then be used to determine (and document consideration of) the appropriate type of agreement.  To request OSP to disburse federal funds awarded after December 26, 2014 as a subaward, send a copy of the completed Third Party Agreement Determination Form, the SOW, and all other Subaward Required Documents (per subaward) to osp@uab.edu. NOTE: The UAB PI and OSP Assigned Number must be included in the email subject line and all documents completed and included in order for the OSP to accept the submission as complete.  If you determine the disbursement should be as a contract (fee-for-service or procurement) include the SOW and Third Party Agreement Determination Form with the other documents you send to University Contracts.
    Effective April 9, 2015, you will be asked to complete the Third Party Agreement Determination Form for any outstanding subawards or subaward requests for any post UG awards received by UAB. If you anticipate or are notified that an award is forthcoming that included a third party in the role of subrecipient you may expedite the process by reviewing the SOW and completing the Form appropriately. Going forward the SOW Template and Form should be used for any subrecipient at preparation of an application or submission in order to determine the appropriate role and costs for the subrecipient.
    Posted by Office of Sponsored Programs | 04/09/15
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