Tissue collectionCollect tissues using sharp scissors or scalpel blades and mouse-tooth forceps. Handle tissues gently. Grasp and cut samples at the edges, so the areas of interest aren't damaged. At right is a section of mouse spleen damaged by rough handling. Many of the cells are distorted beyond recognition.FixationThere are many fixatives and many considerations in their use. For routine paraffin processing and most stains, neutral buffered 10% formalin is a good choice. In general, mouse tissues should be fixed in formalin for 24 to 48 hours, but can be left much longer. Our standard fixative for rodent tissues is 75% ethanol-10% formalin. The ethanol speeds fixation and stiffens tissues such as lung for easier trimming. Bouin's is good for mouse embryos and fetuses, but is not as forgiving, and the tissues must be adequately rinsed after fixing to remove the picric acid. Fixatives can be purchased from vendors of scientific supplies. Contact us if you're not sure what fixation protocol to use.Use plenty of fixative!In general, the ratio of fixative to tissue (vol:vol) should be 15:1 to 20:1. Dense (skin, bone) and bloody (liver, spleen) tissues are hardest to fix. If the amount of fixative is inadequate, or the sample is too large or too thick, the interior of the specimens won't be properly fixed. Inadequate fixation is one of the most common problems we encounter.Fresh tissues tend to stick together and to the bottom of the container, which inhibits fixation. Thus, it's a good habit to swirl the containers a time or two to separate the tissues from each other and from the container bottom. If there is a lot of blood in the fixative after tissue collection, replace it with fresh fixative.Tissues must be placed in fixative promptly to prevent autolysis. Refrigerating the fixative and specimen containers slows autolysis. Sometimes additional procedures are needed. For example, lungs might need to be infused with fixative to approximate the normal state of inflation, and intestines may need to be injected with fixative or opened for optimal fixation. At right is a section of mouse large intestine in which the mucosal morphology is lost due to autolysis.
The Office of Sponsored Programs is pleased to announce the launch of our new OSP website. The new OSP website offers users a more efficient navigational design structure with enhanced content categories.
For more information about changes to the new OSP website, please visit our welcome page. If you currently have forms or pages from our existing website bookmarked, these links should be automatically redirected to the new the website content.
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Center for Center for Disability Health and Rehabilitation Science presents
Professor Robert W. Motl, PhDDepartment of Kinesiology and Community Health Neuroscience ProgramUniversity of Illinois at Urbana - Champaign:
Physical Activity and Multiple Sclerosis - What We’ve Learned and Where We Need To Go
Thursday, April 14, 2016 12:00-1 p.m.School of Health Professions Building Executive Learning Center, Room 640
Bring Your Lunch
Beverages will be provided
To RSVP, please contact Jane Moore at (205) 934-5909 or Email: firstname.lastname@example.org no later than Noon Wednesday 4/13
The Material Transfer Office is changing the process for providing finalized agreements to the campus. Previously the finalized documents were emailed to the investigator, now the investigator will be notified that the finalized agreement is available for viewing through an eReport.
Details can be found on the IRAP Website.