Tissue collectionCollect tissues using sharp scissors or scalpel blades and mouse-tooth forceps. Handle tissues gently. Grasp and cut samples at the edges, so the areas of interest aren't damaged. At right is a section of mouse spleen damaged by rough handling. Many of the cells are distorted beyond recognition.FixationThere are many fixatives and many considerations in their use. For routine paraffin processing and most stains, neutral buffered 10% formalin is a good choice. In general, mouse tissues should be fixed in formalin for 24 to 48 hours, but can be left much longer. Our standard fixative for rodent tissues is 75% ethanol-10% formalin. The ethanol speeds fixation and stiffens tissues such as lung for easier trimming. Bouin's is good for mouse embryos and fetuses, but is not as forgiving, and the tissues must be adequately rinsed after fixing to remove the picric acid. Fixatives can be purchased from vendors of scientific supplies. Contact us if you're not sure what fixation protocol to use.Use plenty of fixative!In general, the ratio of fixative to tissue (vol:vol) should be 15:1 to 20:1. Dense (skin, bone) and bloody (liver, spleen) tissues are hardest to fix. If the amount of fixative is inadequate, or the sample is too large or too thick, the interior of the specimens won't be properly fixed. Inadequate fixation is one of the most common problems we encounter.Fresh tissues tend to stick together and to the bottom of the container, which inhibits fixation. Thus, it's a good habit to swirl the containers a time or two to separate the tissues from each other and from the container bottom. If there is a lot of blood in the fixative after tissue collection, replace it with fresh fixative.Tissues must be placed in fixative promptly to prevent autolysis. Refrigerating the fixative and specimen containers slows autolysis. Sometimes additional procedures are needed. For example, lungs might need to be infused with fixative to approximate the normal state of inflation, and intestines may need to be injected with fixative or opened for optimal fixation. At right is a section of mouse large intestine in which the mucosal morphology is lost due to autolysis.
UAB’s template confidential disclosure agreements have been updated and are available at the OSP Confidentiality Disclosure Agreement (CDA) webpage. The following confidential disclosure agreement (CDA) templates are available:
Mutual Confidential Disclosure Agreement (Company and UAB disclosing confidential information);
Unilateral Confidential Disclosure Agreement (Company Disclosing to UAB); and
Unilateral Confidential Disclosure Agreement (UAB Disclosing to Company)
Please use, and encourage the contracting party to use, the appropriate UAB template CDA. If the contracting party can agree to the terms of one of UAB’s template CDAs, UAB will sign the agreement without further routing and review, resulting in a more expeditious process.
Please forward any questions regarding the CDA process, or UAB’s template CDAs to email@example.com.
On April 30, 2015 the Form FDA 1572 on the FDA website at http://www.fda.gov/AboutFDA/ReportsManualsForms/Forms/ListFormsAlphabetically/ is expired.
The FDA is in the process of updating and posting a new Form FDA 1572. Even though it is expired, the FDA approved continued use of the current form.
We will monitor the FDA website and will post notice of the new form's release, when available.
The Office of Sponsored Programs is pleased to announce additional enhancements to the OSP dropbox for electronic SF424 grant applications to grants.gov. The link to access the OSP dropbox is OSP Drop Box for Electronic Submissions to Grants.gov . The revised OSP dropbox user guide can be found at OSP Dropbox Quick Submission Guide. New enhancements to the dropbox include:
The attribute page has been modified to prompt the user to create a record and then upload the files. When the proposal record is created the attribute page is refreshed with the comment “Submit Successful*” with a footnote to continue to upload files (“*Please continue on to the Upload Files section and attach your proposal documents”).
Once the proposal record is created, the Upload file tab is now used to upload the proposal documents.
The upload file history can now be viewed using the “File History” section. This allows users to verify that the upload was successful.
If you have any questions regarding the new OSP dropbox functionality, please contact your OSP federal officer or firstname.lastname@example.org.