Use a sharp blade such as this one, which has a handle with replaceable blades. Handles and blades are available from vendors of histology and pathology supplies.Tissues must be trimmed to a thickness that will allow them to be completely permeated with paraffin, and to provide the orientation needed. We suggest "thick as a nickel" as a thumb rule. Some mouse organs are small enough that they are best embedded whole.
If there is an area of particular interest, such as a tumor, in the specimen, trim the tissue so that the area of interest is near one surface of the specimen, and place that surface down in the cassette.Be sure to let the histotechs know if the area of interest is very small to ensure that it is not lost or missed. Be advised that it is very difficult to trim and embed tissues so precisely that it can be determined before sectioning which sections will contain such areas. In such cases it is advisable to request serial sectioning and to save all sections until you're certain you've obtained the information you need. Blocks can always be cut deeper, but discarded sections are gone forever.Don't place too many tissues in the cassette. Overcrowding can inhibit proper processing, and it isn't possible to get that many tissues positioned in the block for good sectioning anyway. This one should have about 1/2 or 2/3 of the amount of tissue shown. As a general rule, place tissues of similar consistency together. For example, don't place tough tissues such as skin in the same cassette as soft tissues such as spleen or brain. If the tissues vary greatly in consistency, they may not all section well.If the tissue is very small, biopsy pads can be used to help prevent the specimen from being lost. Biopsy cassettes with fine screens also are available. Biopsy pads also can be used to prevent samples of skin and hollow organs from curling during fixation, but care must be taken not to compress or distort the tissue.In general, biopsy pads should not be used with larger tissue samples. However, they can be used to maintain orientation or positioning of trimmed samples, as distortion is of less concern with fixed solid tissues. (See the examples on the Special processing & embedding page.)
The Office of Sponsored Programs is pleased to announce the launch of our new OSP website. The new OSP website offers users a more efficient navigational design structure with enhanced content categories.
For more information about changes to the new OSP website, please visit our welcome page. If you currently have forms or pages from our existing website bookmarked, these links should be automatically redirected to the new the website content.
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Center for Center for Disability Health and Rehabilitation Science presents
Professor Robert W. Motl, PhDDepartment of Kinesiology and Community Health Neuroscience ProgramUniversity of Illinois at Urbana - Champaign:
Physical Activity and Multiple Sclerosis - What We’ve Learned and Where We Need To Go
Thursday, April 14, 2016 12:00-1 p.m.School of Health Professions Building Executive Learning Center, Room 640
Bring Your Lunch
Beverages will be provided
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The Material Transfer Office is changing the process for providing finalized agreements to the campus. Previously the finalized documents were emailed to the investigator, now the investigator will be notified that the finalized agreement is available for viewing through an eReport.
Details can be found on the IRAP Website.
Richard B. Marchase, Ph.D.
Vice President for Research and Economic Development
1720 2nd Avenue South
Birmingham, AL 35294-0111
Any submissions in progress, in route or received by the OSP, will be updated to reflect the correct signing official.
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