Use a sharp blade such as this one, which has a handle with replaceable blades. Handles and blades are available from vendors of histology and pathology supplies.Tissues must be trimmed to a thickness that will allow them to be completely permeated with paraffin, and to provide the orientation needed. We suggest "thick as a nickel" as a thumb rule. Some mouse organs are small enough that they are best embedded whole.
If there is an area of particular interest, such as a tumor, in the specimen, trim the tissue so that the area of interest is near one surface of the specimen, and place that surface down in the cassette.Be sure to let the histotechs know if the area of interest is very small to ensure that it is not lost or missed. Be advised that it is very difficult to trim and embed tissues so precisely that it can be determined before sectioning which sections will contain such areas. In such cases it is advisable to request serial sectioning and to save all sections until you're certain you've obtained the information you need. Blocks can always be cut deeper, but discarded sections are gone forever.Don't place too many tissues in the cassette. Overcrowding can inhibit proper processing, and it isn't possible to get that many tissues positioned in the block for good sectioning anyway. This one should have about 1/2 or 2/3 of the amount of tissue shown. As a general rule, place tissues of similar consistency together. For example, don't place tough tissues such as skin in the same cassette as soft tissues such as spleen or brain. If the tissues vary greatly in consistency, they may not all section well.If the tissue is very small, biopsy pads can be used to help prevent the specimen from being lost. Biopsy cassettes with fine screens also are available. Biopsy pads also can be used to prevent samples of skin and hollow organs from curling during fixation, but care must be taken not to compress or distort the tissue.In general, biopsy pads should not be used with larger tissue samples. However, they can be used to maintain orientation or positioning of trimmed samples, as distortion is of less concern with fixed solid tissues. (See the examples on the Special processing & embedding page.)
UAB’s template confidential disclosure agreements have been updated and are available at the OSP Confidentiality Disclosure Agreement (CDA) webpage. The following confidential disclosure agreement (CDA) templates are available:
Mutual Confidential Disclosure Agreement (Company and UAB disclosing confidential information);
Unilateral Confidential Disclosure Agreement (Company Disclosing to UAB); and
Unilateral Confidential Disclosure Agreement (UAB Disclosing to Company)
Please use, and encourage the contracting party to use, the appropriate UAB template CDA. If the contracting party can agree to the terms of one of UAB’s template CDAs, UAB will sign the agreement without further routing and review, resulting in a more expeditious process.
Please forward any questions regarding the CDA process, or UAB’s template CDAs to email@example.com.
On April 30, 2015 the Form FDA 1572 on the FDA website at http://www.fda.gov/AboutFDA/ReportsManualsForms/Forms/ListFormsAlphabetically/ is expired.
The FDA is in the process of updating and posting a new Form FDA 1572. Even though it is expired, the FDA approved continued use of the current form.
We will monitor the FDA website and will post notice of the new form's release, when available.
The Office of Sponsored Programs is pleased to announce additional enhancements to the OSP dropbox for electronic SF424 grant applications to grants.gov. The link to access the OSP dropbox is OSP Drop Box for Electronic Submissions to Grants.gov . The revised OSP dropbox user guide can be found at OSP Dropbox Quick Submission Guide. New enhancements to the dropbox include:
The attribute page has been modified to prompt the user to create a record and then upload the files. When the proposal record is created the attribute page is refreshed with the comment “Submit Successful*” with a footnote to continue to upload files (“*Please continue on to the Upload Files section and attach your proposal documents”).
Once the proposal record is created, the Upload file tab is now used to upload the proposal documents.
The upload file history can now be viewed using the “File History” section. This allows users to verify that the upload was successful.
If you have any questions regarding the new OSP dropbox functionality, please contact your OSP federal officer or firstname.lastname@example.org.