Use a sharp blade such as this one, which has a handle with replaceable blades. Handles and blades are available from vendors of histology and pathology supplies.Tissues must be trimmed to a thickness that will allow them to be completely permeated with paraffin, and to provide the orientation needed. We suggest "thick as a nickel" as a thumb rule. Some mouse organs are small enough that they are best embedded whole.
If there is an area of particular interest, such as a tumor, in the specimen, trim the tissue so that the area of interest is near one surface of the specimen, and place that surface down in the cassette.Be sure to let the histotechs know if the area of interest is very small to ensure that it is not lost or missed. Be advised that it is very difficult to trim and embed tissues so precisely that it can be determined before sectioning which sections will contain such areas. In such cases it is advisable to request serial sectioning and to save all sections until you're certain you've obtained the information you need. Blocks can always be cut deeper, but discarded sections are gone forever.Don't place too many tissues in the cassette. Overcrowding can inhibit proper processing, and it isn't possible to get that many tissues positioned in the block for good sectioning anyway. This one should have about 1/2 or 2/3 of the amount of tissue shown. As a general rule, place tissues of similar consistency together. For example, don't place tough tissues such as skin in the same cassette as soft tissues such as spleen or brain. If the tissues vary greatly in consistency, they may not all section well.If the tissue is very small, biopsy pads can be used to help prevent the specimen from being lost. Biopsy cassettes with fine screens also are available. Biopsy pads also can be used to prevent samples of skin and hollow organs from curling during fixation, but care must be taken not to compress or distort the tissue.In general, biopsy pads should not be used with larger tissue samples. However, they can be used to maintain orientation or positioning of trimmed samples, as distortion is of less concern with fixed solid tissues. (See the examples on the Special processing & embedding page.)
The UAB Office of Sponsored Programs is pleased to announce the newly revised procedure for review of NIH proposal submissions for large submission deadlines involving NIH Research Project Grants (R01), Research Career Development Awards (K) and Research Fellowship (F) proposals. In order to review NIH proposals as quickly as possible, OSP has implemented a proposal review pool which will allow OSP to introduce additional reviewers to assist with proposal review when needed.
NIH proposal submissions to OSP for deadline dates of June 5th and July 5th (R01), June 12th and July 12th (K) and August 8th and 13th (F), will be assigned rotating through a pool of reviewers instead of an assignment to a specific officer by department. The reviewer will be identified in the Receipt of Submission email once a complete submission is received by OSP. This change is effective immediately.
NOTE: This is only applicable to new NIH proposal submissions for the above mentioned NIH deadlines. All other NIH proposal submission deadlines will be routed to the officer assigned to your specific department. All NIH awards will continue to be processed by the OSP officer assigned to your specific area.
Please continue to follow the OSP Review Plan to ensure that your reviewer has sufficient time to properly review the proposal.
Please forward any questions regarding the NIH proposal review process to Tim Parker at email@example.com or firstname.lastname@example.org.
The Office of Sponsored Programs is pleased to announce the launch of our new OSP website. The new OSP website offers users a more efficient navigational design structure with enhanced content categories.
For more information about changes to the new OSP website, please visit our welcome page. If you currently have forms or pages from our existing website bookmarked, these links should be automatically redirected to the new the website content.
For additional information, please contact Mike Matthews at email@example.com or Jonathan Price at firstname.lastname@example.org.
Center for Center for Disability Health and Rehabilitation Science presents
Professor Robert W. Motl, PhDDepartment of Kinesiology and Community Health Neuroscience ProgramUniversity of Illinois at Urbana - Champaign:
Physical Activity and Multiple Sclerosis - What We’ve Learned and Where We Need To Go
Thursday, April 14, 2016 12:00-1 p.m.School of Health Professions Building Executive Learning Center, Room 640
Bring Your Lunch
Beverages will be provided
To RSVP, please contact Jane Moore at (205) 934-5909 or Email: email@example.com no later than Noon Wednesday 4/13