Animal Physiology Core

The Animal Physiology Core (APC) provides for diabetes related phenotyping in small animal models. Services offered include the assessment of body composition, energy balance, glucose homeostasis, cardiovascular assessment, imaging, and transgenic animals models.


Objectives

Objectives

The objectives of the core are to provide:

  • Body composition: Whole-body composition analysis by chemical carcass analysis, dual-energy X-ray absorptiometry (DXA), quantitative magnetic resonance (QMR), and micro-computed tomography (µCT)
  • Energy balance: Comprehensive assessments of metabolic rate (indirect calorimetry), food intake, fecal output, activity, and body temperature
  • Glucose homeostasis: Glucose and insulin tolerance testing
  • Cardiovascular assessment: Echocardiography and blood pressure
  • Imaging: Bioluminescence and fluorescence imaging and gamma-ray imaging, including SPECT
  • Transgenic animal models: Assistance with construct preparation, generation of transgenic/knock-out mice, husbandry and colony management, and genotyping

Services

Services

The APC emphasizes quality control in all aspects of its functions. All data is examined and quality controls performed in a manner that is specific for the multiple core methodologies. Aspects of these procedures are discussed below in the context of provided core services.

1. Whole-body composition analysis

  • Chemical carcass analysis (CCA)
  • Dual-energy X-ray absorptiometry (DXA)
  • Quantitative magnetic resonance (QMR)
  • In vivo and ex vivo imaging of small rodents using micro-computed tomography (µCT)
  • Measurement of in vivo liver fat using µCT

2. Comprehensive assessments of metabolic rate (indirect calorimetry), food intake, fecal output, and activity

3. Glucose homeostasis

  • Insulin tolerance test (ITT)
  • Glucose tolerance test (GTT)

4. Cardiovascular assessments

  • Systolic function
  • Diastolic function
  • Vascular compliance
  • Blood pressure

5. Optical Imaging

  • Bioluminescence and fluorescence
  • Gamma-ray imaging, including SPECT

6. Transgenic Animal/Embryonic Stem Cell Resource

  • Expertise related to transgenics.
  • Services Offered by the UAB Transgenic Mouse Facility
    • DNA microinjection (pronuclear)
    • Gene Targeting of ES cells (with & without screening)
    • ES cell microinjection (blastocysts)
    • In vitro fertilization (IVF)
    • Embryo cryopreservation
    • Sperm cryopreservation
    • Long-term storage of cryopreserved transgenic lines
    • Assisted reproduction / rederivations
    • Consultation & training


Personnel

Personnel

Timothy

Director
Timothy R. Nagy, Ph.D.

Webb 419
1675 University Blvd
Birmingham, AL 35294
Phone: (205) 934-4088
Fax: (205) 934-7050
Email: tnagy@uab.edu

Yang

Co-Director
Qinglin Yang, M.D., Ph.D.

Webb 435
1675 University Blvd
Birmingham, AL 35294
Phone: (205) 996-6022
Fax: (205) 934-7049
Email: qyang@uab.edu

Kurt

Co-Director
Kurt Zinn, M.S., D.V.M., Ph.D.

VH G082
1670 University Blvd
Birmingham, AL 35294
Phone: (205) 975-6414
Fax: (205) 975-6522
Email: kurtzinn@uab.edu
Robert

Co-Director
Robert A. Kesterson, Ph.D.

Kaul Human Genetics Bldg Room 602A
720 20th Street South
Birmingham, AL 35294
Phone: (205) 934-7206
Fax: (205) 975-4418
Email: kesterso@uab.edu