About the Facility: 

The Multidisciplinary Molecular Interaction Core (MMIC) provides use of a Biacore T200 biosensor that is capable of determining the binding kinetics, specificities, and thermodynamics of biomolecular interactions.

The Biacore T200 uses surface plasmon resonance (SPR) technology to precisely monitor label-free binding activity between proteins, peptides, nucleic acids, carbohydrates, lipids or small drugs (200-1000 Daltons) in real-time. Biacore T200 biosensor was purchased through NIH support, grant S10 RR026935. For more details of the Biacore technologies go to http://www.biacore.com/lifesciences/index.html.

Multidisciplinary Molecular Interaction Core (MMIC) is located in Shelby Building room 420. The core is directed by PI, Randall Davis, M.D. and managed and operated by Edlue M. Tabengwa, Ph.D. The facility supports a GE Biacore T200 optical biosensor instrument that employs surface plasmon resonance (SPR) technology for label-free, real-time, high-quality comprehensive characterization of biomolecular interactions including proteins, nucleic acids, carbohydrates, lipids, and viruses. This instrumentation provides determinations of binding specificities, kinetics, affinity, physiologic concentrations previously beyond detection limits, interacting partners with low molecular weights, immunogenicity, thermodynamics, and epitope mapping. This facility supports the work of eight major user PIs on a fee for service basis. The availability of this instrumentation and core is an outstanding resource for antibody validation and authentication, as well as drug and ligand studies.

BiaCore T200