Gene Expression
Gene expression assays for targeted arrays of genes or whole genomes are available for interrogation. Expression assays involve synthesis and labeling of double-stranded cDNA from extracted RNA, which is then concentrated, fragmented, and hybridized to a BeadChip fiber optic bundle or chip array containing sequences representing up to 48,000 known genes and ESTs.
| Illumina | Affymetrix |  |
| Human HT-12 v4 |
Human Exon 1.0 ST Array | |
| Mouse WG-6 v2 | Human Gene 1.0 ST Array | |
| Mouse Ref-8 v2 | Human Genome U133 Plus 2.0 Array |
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| miRNA |
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| Mouse Exon 1.0 ST Array | ||
| Mouse Gene 1.0 ST Array | ||
| Mouse Genome 430 2.0 Array | ||
| Rat Exon 1.0 ST Array |
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| Rat Gene 1.0 ST Array | ||
| Rat Genome 230 2.0 Array | ||
| Other Organisms |
Gene expression assays for targeted arrays of genes or whole genomes are also available. The Core Facility maintains the Roche LightCycler 480 Real Time PCR machine for allelic discrimination.