Thermo Fisher Orbitrap Velos Pro

Dedicated to Leon Edwards of "Edwards Chevrolet" for his generous donation leading to the purchase of this instrument for cancer research

 LeonEdwardsEditedOrbitrapVelosPro



Thermo Fisher Orbitrap Velos Pro

Agilent 1260 infinity nano-HPLC stack and Auto-Sampler

ThermoAgilent



Thermo Fisher LTQ-XL

Dionex Ultimate 3000 UHPLC+ Focused Stack & Auto Sampler

ThermoDionex


Thermon Fisher LTQ-XL with ETD

Surveyor MS HPLC Stack & Auto-Sampler

thermosurveyor


Bruker UltraFlex III MALDI ToF/ToF

bruker


Shimadzu ChIP-1000 (HD Chemical Printer for Imaging-MS)

Shimadzu


GE Ettan™ IPGphor IEF System

Large Format BioRad Protean® Plus Dodeca Cell w/ Power Supply

Amersham Multitemp III w/ Circulating Pump

GEEttanBioRadAmersham

GE Ettan™ Spot Picker

GEEttan


BioTek Microplate Spectrophotometer

(Synergy 2 Multi-Mode UV/ VIS/ / NIR/ FL/ L Top&Bottom Reader)

BioTek


UVP BioSpectrum® Imaging System (UV/ VIS/ IR/ FL/ L) 

 UVP


Shandon Cryotome™ FSE Cryostat w/ Histology Station 

 Shandon


Sutton Nano Laser Quartz Tip Puller w/ column packing bomb 

 Sutton


GE nanoCOLLECT System (LC-MALDI-MS Spotter)

Surveyor MS HPLC Stack & Auto-sampler

 GESurveyor


Shimadzu (analytical/ preparative) HPLC w-UV/Vis Detector

Gilson Fraction Collector

 ShimadzuGilson


Cell Culture Room: Laminar Flow Hood, CO2 Incubator, & 2 Microscopes

 Cell Culture Room


Two -80°CRefrigerators (Long-Term Storage Available For MSP-SF Users)

 Two 80C Refrigerators


Server Stack

 ServerStack


Our facility houses nearly 30 computers that include 2 high-end workstations and 5 high-speed high capacity double firewalled servers(below) that contain 40TB of dynamic drive space and an additional 60TB of backup storage for archiving and automated nightly backups for all computers throughout the MSP-SF (including all MS instruments).

  • Primary fileserver: (30TB of drive space, 32 GB RAM, 2 . AMD 3.1 GHz 8 Core processors)
  • Unix application server: powered by an Oracle Database (64 GB RAM, 2 . AMD 2.6GHz 12 Core processors)
  • Primary domain controller: for our network (2 GB RAM, 1 . Intel 2.00 GHZ Dual Core processor)
  • Secondary fileserver: (4 GB RAM, 2 . Intel 2.00 GHz Dual Core Processors)
  • Application server: (32 GB RAM, 2 . AMD 2.2GHZ 12 Core processors)

The primary fileserver is also dynamically linked to a 2000 node Cluster - UAB Shared High Performance Computing Facility. (https://docs.uabgrid.uab.edu/wiki/Resources)

Software:

  • Search Engines: SEQUEST, MASCOT
  • Denovo Sequencing: MASCOT Distiller
  • 2D PAGE Analysis: Nonlinear Samespots
  • MS Peptide & PTM Grouping, Filtering, and Quantification Analysis: MASCOT Distiller Q+, Proteome Software Scaffold, Scaffold PTM/ Q+S
  • Bruker Daltronics Suite: LCWARP, Biotools, Rapid Denovo, Fleximaging
  • Statistical Analysis: Matlab, Statistica, SPSS, Graphpad, Origin
  • Systems Analysis: GeneGO-metaCore, Blast2Go-Pro
  • Data Sharing: MSP-SF LIMS, all data is retrievable via HTML

Additional Essential Equipment:

  • Small Molecule & Lipid Preparation Station (including two multi-well SPE devices w/ multi-well heated drying system – w/ argon & nitrogen)
  • Chemical Synthesis & Distillation Apparatus
  • Savant Explorer SpeedVac® Concentrator with multiple adapters including 96 deep well
  • Sorvall Legend XTR centrifuge with multiple adapters including a 96 deep well plate holder
  • Chemical Fume Hoods (2)
  • Refrigerated Cold Cabinets (2)
  • Freezers -20°C (2)
  • Multiple Centrifuges (cooled and RT)
  • Multiple Standard and Midi gel boxes for 1D PAGE & WB
  • 1D Gel Electro-Eluter
  • Thermocyclers (3)
  • High-Speed 96 Multi-Well Shaker
  • Heated Multi-Well Shaker
  • Large Water Bath Sonicator
  • Cell Disruptor