We are happy to assist you and look forward to working with you. To schedule a free initial 15-minute Zoom consultation, request a letter of support, inquire about a service/training or any other request, please use this link or scan the QR code:

Service Request Form

BARB Core Lab Clean Up & Close Out Services

1. To request our lab clean-up or close-out services, please fill out the service request form using this link: https://forms.office.com/r/6mDGk2XYuY
2. Once we receive the request, we will schedule a free 15-min Zoom consultation where we can discuss and coordinate the services requested.
3. Then we will contact EH&S on your behalf and notify them of the intent to move/closeout these lab spaces, tag out equipment, and schedule a tour of the lab spaces with an EH&S Research Safety Representative to provide guidance on proper procedures to be followed.
4. Then we will tour the spaces together, and my team can begin the clean out.

We are fully certified by UAB EH&S to do:

• biohazard waste removal
• chemical segregating/packing/manifesting
• equipment inventorying, decontaminating, and tagging for surplus (usually offered up to other PIs in the department first, along with consumables)
• cleaning and boxing up for donation to Green Labs (offered to department PIs first)
• closing out labs

Our services are first-come, first-serve. We would prefer to have as much notice as you can give us, but if you have a deadline or special circumstances, we will do our best to expedite the process. We typically have 3 team members who work on these cleanouts. Our fees are $100/h per team member. If all 3 of us work at once, we get it done 3x as fast, but the total cost remains the same as if one of us worked and it takes 3x as long. This allows us to be flexible and get the clean-ups/clean-outs done as efficiently as possible.

See what the UAB community has to say about working with the BARB Core Team to clean up and close out labs across campus: https://www.uab.edu/reporter/in-the-know/uab-barb-core-handles-the-tough-tasks-of-cleaning-out-labs

Before

After

Publications: dietary inflammation, discrimination and stress, chronic cortisol in adult cancer survivors, gardening intervention trial in cancer survivors.
 

Sample: frozen serum/plasma (human, mouse)

Equipment: Applied Biosystems StepOnePlus Real-Time PCR System, Applied Biosystems DynaMag Magnet, Biotek Synergy 2 Multi-Mode Microplate Reader, LabNet Plate Spinner

Publications: HIV and Chronic Pain
 

• Complex I (NADH:Ubiquinone Oxidoreductase) Assay
• Complex II (Succinate-Ubiquinone Oxidoreductase) Assay
• Complex III (Decylubiquinol Cytochrome C oxidoreductase) Assay
• Complex IV (Cytochrome c oxidase) Assay
• Complex V (ATP hydrolysis) Assay
• Citrate Synthase Assay

Sample: fresh or frozen tissues or cells (human, rodent, fish, Drosophila)

Equipment: Beckman DU 800 Spectrophotometer, Custom-Built Mitochondrial Isolation Stations with Recirculating Chiller

Training: Please contact us to schedule

Publications: skeletal muscle in rheumatoid arthritis, TrxR2 and glucose tolerance, neonatal hippocampus development, brain IGF-1 and aging, mitochondrial fusion and exercise
 

• High Resolution Respirometry (HRR) - Oxidative Phosphorylation ONLY
  

Sample: fresh, permeabilized, ex vivo tissues (human muscle, rodent renal cortex, medulla, and microvessels, embryonic and adult heart, gastroc and soleus muscle, hippocampus, bladder, liver) or isolated mitochondria (rodent, fish, Drosophila)

Equipment: 3 x Oroboros Oxygraph-2k Fluorometers (LED2-Module Amperometric Add-On)

Training: Please contact us to schedule

Publications: neonatal lung injury, skeletal muscle in rheumatoid arthritis, embryonic heart cardiogenesis, soleus muscle in spinal cord injury, post-exercise skeletal muscle, neonatal hippocampus development, aerobic exercise training

• Seahorse Extracellular Flux Analysis (XFe96) – Oxidative Phosphorylation and Glycolysis
  

Sample: cultured cells or freshly isolated mitochondria (human, rodent), mycoplasmas.

Equipment: Agilent Seahorse XFe96, high throughput, 96-well format

Training: Please contact us to schedule

Publications: neonatal lung injury, ovarian cancer, breast cancer, bronchopulmonary dysplasia, adrenal neuroendocrine cancer, glucose tolerance, mammary tumor-associated macrophages, diabetic retinopathy
 

• For High Resolution Respirometry (HRR)
• For Mitochondrial Electron Transport Chain (ETC) Kinetics

• Catalase
  Sample: plasma, serum, total homogenate

• Glutathione Tietze (GSSG and GSH)
  Sample: plasma, whole blood

• Glutathione Peroxidase (GPX)
  Sample: plasma, total homogenate

• Total Antioxidant Capacity (TAC)
  Sample: plasma, serum, urine, saliva

• Ferric Reducing Antioxidant Power (FRAP)
  Sample: plasma