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  • Picture 1
    Cryo-Electron Microscopy Facility

  • Thermo Scientific Glacios 2
    Thermo Scientific Glacios 2

    Thermo Scientific Glacios 2

    Overview:

    The Glacios 2 cryo-TEM is optimized for high-throughput screening, single-particle data collection, and cryo-electron tomography (cryoET) of cryogenic samples.

    Features:
    • Falcon 4i direct electron detector
    • Ceta 16M CMOS camera
    • SmartEPU with Quality Monitor, Multigrid, and
    • Tomography 5
    • 12-sample Autoloader
    • Aberration-free image shift (AFIS)
    • Fringe-free imaging (FFI)
    Imaging Parameters:
    • Accelerating voltage: 200kV
    • Spherical aberration (Cs): 2.7mm
    Normal Magnification Pixel Size (Å/pix) Benchmark Resolution (Å)+
    73,000x 1.92
    92,000x 1.53
    120,000x 1.166** 2.64
    150,000x 0.94
    190,000x 0.7159** 2.10

    * 190,000 x magnification maximizes the field of view relative to the area exposed with the electron beam, covering the greatest area on the sample grid in the shortest time possible.

    ** Pixel sizes calibrated by comparison of a benchmark apoferritin reconstruction to a high-resolution x-ray crystal structure. Other pixel sizes are measured using a standard cross-grating replica grid.

    + Single-particle reconstruction of equine spleen apoferritin (Sigma Aldritch).

    Learn More

  • Talos F200C G2 (Coming Soon)
    Talos F200C G2 (Coming Soon)

    Talos F200C G2 (Coming Soon)

    Features:

    Side entry sample port
    Long duration Dewar
    EPU software
    Tomography Data Acquisition Software
    MicroED software
    Ceta S camera

    Imaging parameters:

    Accelerating voltage: 200kV
    Cs: 2.7mm

  • FEI Vitrobot Mark IV
    FEI Vitrobot Mark IV

    FEI Vitrobot Mark IV

  • PIE Scientific Tergeo EM Plasma Cleaner
    PIE Scientific Tergeo EM Plasma Cleaner

    PIE Scientific Tergeo EM Plasma Cleaner

  • Pelco EasiGlow Glow Discharge System
    Pelco EasiGlow Glow Discharge System

    Pelco EasiGlow Glow Discharge System

  • Sample Holders

    Sample Holders

    Gatan 626 cryo sample holder

    Gatan 698 (Elsa) cryo sample holder