HRIF reopening.

HRIF reopens Monday June 1st.

Dear HRIF users,
The High Resolution Imaging Facility is going to be open for business beginning Monday June 1. We will be opening under a new model. Current HRIF users will receive an email with new operations protocol explaining in details our new rules. If you did not receive the email you can request it from HRIF personnel. Please familiarize yourself with the new SOP BEFORE coming to the core.
Thank you.

Lionheart FX is here!

Lionheart FX is here!

Lionheart FX is here!

January, 2020

We are very pleased to announce a new and exciting addition to our HRIF core; Lionheart FX Automated Imaging System from Biotek. This small footprint yet mighty microscope offers a broad variety of high quality imaging techniques, including:
  • Fluorescent, color or black and white brightfield, and phase-contrast
  • High speed fluorescent imaging of up to four channels with laser autofocus
  • Single image or z-stacks
  • Image stitching of fluorescent and brightfield specimen
  • Environmental control for live imaging

The Gen5 software, also available on our stand alone workstation Analysis PC, allows for a broad range of image rendering and analysis. Now you will be able to:
  • Measure confluency on single or time-lapse images
  • Count label-free cells
  • Measure kinetics of cell migration, scratch invasion, and spheroid formation
  • Nuclei count, cytoplasm and organelle analysis
  • Monitor and analyze molecular movement between cellular compartments
  • And more…

The system offers five air objectives at 1.25, 4, 10, 20 and 60x magnification that will accommodate most basic imaging needs. And don’t forget, our friendly staff is always here to help.

New TEM Camera

New TEM Camera

New High Speed TEM Camera

October 08, 2019

We are pleased to announce a recent upgrade in the Transmission Electron Microscopy Lab in the HRIF. Our TEM Lab (located in the Shelby Building Basement in Suite B40) has acquired an Advanced Microscopy Techniques (AMT) BioSprint 29, a high speed 29 Megapixel TEM camera which employs the use of AMT’s high precision ActiveVu lens. This system provides high definition images that are perfect for any life science application. The new addition of the camera to our existing transmission electron microscope allows investigators to capture images in the magnification range of 18.5x-650kx.

This upgrade was generously funded by UAB’s Health Services Foundation(HSF) and is due to the kind support of investigators like you. We look forward to any feedback on our newly installed system and appreciate your continued support as we apply for future grants so that we can continue to offer technology in support of your research. To schedule imaging time on our new camera, please contact Melissa Chimento or Ed Phillips at 205-934-1926.

Core Day 2019

Core Day 2019

UAB Core Day 2019

July 30, 2019

Hello core users, we are happy to announce that we will be at UAB's Core Day this year on August 28th, 2019 from 11AM to 1PM in the West Pavilion Atrium. Stop by to learn more about what HRIF can do for your research and check out all the other amazing resources available here at UAB!

Core Day website

Leica Demo

Leica Demo

DEMO: Leica Microsystems Confocal Innovations

July 09, 2019

We would like to announce that Leica Microsystems will be here at UAB offering demos on their machines from July 16-25.

Please RSVP for sample preparation information and to reserve your time on the instrument!


Please email Shawn Williams ( if you have any questions or would like to setup a time to see a demonstration.

HRIF Director
Alexa Mattheyses, PhD
Assistant Professor, Department
of Cell, Developmental, and Integrative Biology 
 IMG 4709 copy
Fluorescence and Light Microscopy
Robert Grabski, PhD
Super-resolution, Confocal Microscopy, Live Cell Imaging
 Robert 3
Shawn Williams
Imaging/Confocal Microscopy, Light Microscopy, Nanosight
Transmission Electron Microscopy (TEM)
Melissa Chimento
Transmission Electron Microscopist, Sample Preparation and Sectioning
Ed Phillips
Transmission Electron Microscopist, Sample Preparation and Sectioning