Dr. Steven J Pittler
Dr. Shu-Zhen Wang
Dr. Vincenzo Guarcello
Location & Contact
Phone: (205) 934 6735
The primary purpose of the Molecular/Cellular Analysis module is to assist the 56 members of the Vision Science Research Center to complete molecular biology and histology related portions of their research. This is accomplished by meeting with the researcher to define the approach to the problem, developing or modifying procedures needed, and completing the project as outlined. The module also exists to provide expertise for training and supervision of graduate students and personnel from other labs in molecular biology and histology procedures and in the use of available laboratory equipment; for solving technical problems for these personnel; and to purchase sufficient equipment and supplies for an efficiently operating lab.
The online scheduler is here! Reserve your instrument time on your computer http://scheduler.vsrc.uab.edu/molbio/
The combination of the Molecular module with Histology is now real, and has become the Molecular and Cellular Analysis Module, located in WORB rooms 424 and 310.
Room 310 houses tools for histology, microscopy, water baths, a centrifuge, DNA and protein electrophoretic apparatus, as well as glassware, tubes, tips, and assorted supplies.
Room 424 is still the place for all molecular biology except the gel & blot apparatus, and now boasts a little bit of counter space.
MBP users are hereby empowered (and encouraged) to turn off the lab lights when they leave.And don't forget to log off Windows.
All MBP Module users should acknowledge P30 EY03039 for funding. If you use the lab at all, please include this acknowledgment in every publication. And send Ginger a copy of the citation. These things will help us continue funding the core lab!
The purpose of the Molecular Biology and Physiology Module is to assist VSRC members with the investigation of gene expression, including but not limited to primer and mutant design, amplification and quantitation of RNA or DNA products, cloning, and other molecular biology techniques. The NEI P30-funded Molecular Biology Module is now up and running. The module is managed by Henry K Fortinberry, who has extensive experience in many aspects of molecular biology. The module is currently located in the Worrell Building, Room 424.
- Training in basic molecular biology techniques and equipment usage
- Technical and experimental design advice
- Primer design
- Assistance with creating mutants
- Gradient or conventional PCR and RT-PCR
- Real-time/quantitative PCR and RT-PCR with up to 5 fluorophores
- Gels and blots, imaging
- Bio-Rad iQ5 Real-Time PCR Detection System
- Typhoon Trio+ Imaging System
- Wescor 5520 Vapor Pressure Osmometer
- NanoDrop Spectrophotometer
- BioRad Criterion Gel & Blot System
Beckman TL100 Ultracentrifuge with TLA 100.3 fixed angle rotor and TLS-55 swinging bucket rotor
Eppendorf 5810 refrigerated centrifuge with several rotors and adpaters for well plates and assorted tube sizes
- Bioptigen Optical Coherence Tomography
- Micron III Fundus Scope
- Mini-spin benchtop centrifuge
- Pipetters, freezer bricks, tube racks, tips, tubes, etc.
- Beacon Designer
- Sample data from the MBP ( click on the photos to enlarge them )
Primer design links: