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Heflin Center for Genomic Sciences

Heflin Center for Genomic Sciences

Welcome to the Heflin Center for Genomic Sciences

Welcome to the Heflin Center for Genomic Sciences (HCGS). The Heflin Center was founded in 2002 as a University Wide Interdisciplinary Research Center (UWIRC) with the goal of enhancing resources to enable UAB investigators to incorporate genetic and genomic approaches in their research.

RT-PCR Scheduler All Other Assays Opens an external link. Sanger Sequencing Opens an external link. Order Primers/Oligos from IDT Opens an external link.

DNA Profiling

This service from the Heflin Center Genomics Core Facility uses the Applied Biosystems AmpFlSTR system to screen 15 different small tandem repeat (STR) markers and the Amelogenin locus for gender determination. This service is intended for Human Cell line identification and is for research purposes only. These STR markers include those genotyped by the ATCC. The service will cost $50/cell line. Please contact the Core for more information or to schedule an assay.

The markers include:

Locus Designation               Chromosome Location                       Alleles Included in Allelic Ladder
D8S1179 8 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
D21S11 21q11.2-q21 24, 24.2, 25, 26, 27, 28, 28.2, 29, 29.2, 30, 30.2, 31, 31.2, 32, 32.2,33, 33.2, 34, 34.2, 35, 35.2, 36, 37, 38
D7S820 7q11.21-22 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
CSF1PO 3p 12, 13, 14, 15, 16, 17, 18, 19
TH01 11p15.5 4, 5, 6, 7, 8, 9.3, 10, 11, 13.3
D3S1358 3p 12, 13, 14, 15, 16, 17 ,18, 19
D13S317 13q22-31 8, 9, 10, 11, 12, 13, 14, 15
D16S539 16q24-qter 5, 8,9, 10, 11, 12, 13, 14, 15
D2S1338 2q35-37.1 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28
D19S433 19q12-13.1 9, 10, 11, 12, 12.2, 13, 13.2, 14, 14.2, 15, 15.2, 16, 16.2, 17, 17.2
vWA 12p12-pter 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
TPOX 2p23-2per 6, 7, 8, 9, 10, 11, 12, 13
Amelogenin X:p22.1-22.3
Y:p11.2		 X,Y
D18S51 18q21.3 D18S51 18q21.3 7, 9, 10, 10.2, 11, 12, 13, 13.2, 14, 14.2, 15, 16, 17, 18,
19, 20, 21, 22, 23, 24, 25, 26, 27
D5S818 5q21-31 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
FGA 4q28 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 26.2, 27, 28, 29, 30, 30.2, 31.2, 32.2,
33.2, 42.2, 43.2, 44.2, 45.2, 46.2, 47.2, 48.2, 50.2, 51.2