Next Generation Sequencing

The Genomics Core offers Next Generation Sequencing (NGS) services at UAB using the Illumina Sequencing Platforms: NextSeq500 and the MiSeq desktop sequencer.

NGS Assays include:

• Whole Genome Sequencing
• Targeted Resequencing
• mRNA-Seq
• microRNA-Seq
• Exome Capture and Sequencing
• ChIP-Seq
• Reduced Representation Bisulfite Sequencing (RRBS)
• 16S rDNA Microbiome Sequencing

Data analysis is being performed through the Heflin Center in collaboration with the CCTS, and the CBSG.

Please contact Mike Crowley (975-2013) to discuss the details and pricing for your NGS project.

Articles:

Here is a really good review when planning RNA-Sequencing experiments:

• RNA Sequencing: The Teenage Years (Nature Review Genetics)

Here is a really good review when planning single-cell RNA-Sequencing experiments:

• Single-Cell RNA-Seq Technologies and Related Computational Data Analysis (Frontiers in Genetics)