The primary, global question that our lab seeks to answer is “how does acute critical illness become chronic critical illness?” When patients become severely critically ill in the Intensive Care Unit (ICU), there is ~25% mortality. Of the 75% that survive their stay in the ICU, ~50% will die in the next 1-2 years. Additionally, there is post-ICU cognitive dysfunction that occurs as a result of critical illness, regardless of mortality during the next two years. This mortality and morbidity are costly to patients in terms of their ability to take care of their families and to society in terms of health-care costs, days of work missed and other burdens. We seek to understand why patients that survive acute critical illness have ongoing morbidity and mortality that severely impairs their life and society-at-large. 

We use a variety of in vivo and in vitro techniques to answer questions in our lab including, but not limited to: 

  • Traumatic Brain Injury Mouse Model 
  • Bacterial Pneumonia Mouse Model
  • Techniques to Measure Acute Lung Injury in Mice
  • Cell Culture
  • Western Clotting 
  • Forster-Resonance Energy Transfer
  • Confocal Fluorescence Microscopy 
  • CRISPR
  • Flow Cytometry
  • Immunoprecipitation 
  • Spectrofluoremetry
  • Electronic Cell Impedance Sensing 
  • Human Sample Cell Isolation and Measurements 

We currently work on two projects that are subsets of the aforementioned larger goals: The Role of Sex Dimorphism in post-TBI Bacterial Pneumonia and Role of amyloid-beta (Aβ) in bacterial pneumonia-mediated lung injury