NU-USC George M. O’Brien Kidney Resource Center (NUGoKidney)
Summary
The Northwestern University-University of Southern California (NU-USC) National Resource Center (NUGoKidney) aims to revolutionize kidney research by integrating physical science advances, such as nanotechnology, proteomics, and bioelectronics, into kidney disease study and treatment. Its Biomedical Resources Core objectives include developing new methods for kidney research, providing widespread access to innovative physical science tools, and fostering community-wide innovation through nanotechnology applications. The integration of physical sciences and kidney research positions the NU-USC NRC as a leading institution in advancing kidney health and therapy.
If you are interested in utilizing any resources from this center, please schedule an initial consultation by emailing nephroHUB@northwestern.edu.
Biomedical Resource Core (BRC)
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Description
Services provide precision targeting of desired payloads to specific cells or sub-regions within the kidney through:
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Services
- Applications of immunomagnetic cell sorting (LEAPFROG Platform)
- Designing in vitro studies
- Designing in vivo studies
- Feasibility assessment
- Fluorophore conjugation with DSPE-PEG
- Identifying pathway for therapy
- Identifying targeting peptides
- In vitro cell viability evaluation - MTS assay/plate reader
- In Vitro Micelles Binding Evaluation
- In Vitro Micelles Binding Evaluation: Confocal Microscopy
- In Vitro Micelles Binding Evaluation: Flow Cytrometry
- In Vitro Micelles Efficacy Evaluation
- In Vitro Micelles Efficacy: Quantification of markers related - qPCR
- In Vitro Micelles Efficacy: Quantification of proteins related - ELISA
- In Vitro Micelles Efficacy: Quantification of proteins related - Western blot
- In vitro studies of micelles with Collecting Duct cells - Cortical collecting duct (M1)
- In vitro studies of micelles with Collecting Duct cells - Cortical collecting duct (Principal cells)
- In vitro studies of micelles with Collecting Duct cells - Inner Medullary
- In vitro studies of micelles with Proximal Tubule cells
- In Vivo Micelles Therapeutic Efficacy : Hematoxylin and Eosin (H&E) Imaging
- In Vivo Micelles Therapeutic Efficacy: Immunohistochemistry (IHC) Imaging
- In Vivo Micelles Therapeutic Efficacy: using Rapid Model
- In Vivo Therapeutic Efficacy:
- In Vivo Therapeutic Efficacy: Guidance
- Incorporation of conjugated siRNA into micelles
- Micelles formation
- Nanocarrier/micelles characterization: Morphology - Transmission electron microscopy (TEM)
- Nanocarrier/micelles characterization: particles size - Dynamic Light Scattering (DLS)
- Nanocarrier/micelles characterization: Polydispersity Index (PDI)- Dynamic Light Scattering (DLS)
- Nanocarrier/micelles characterization: RNA incorporation - Gel Electrophoresis
- Nanocarrier/micelles characterization: RNA protection against nucleases - Gel Electrophoresis
- Nanocarrier/micelles characterization: zeta potential - Dynamic Light Scattering (DLS)
- NW Background search
- NW Grant proposal assistance
- NW Manuscript assistance
- NW Publication Assistance
- Peptide purification
- Peptide purification: High-Performance Liquid Chromatography (HPLC)
- Peptide purification: Matrix-Assisted Laser Desorption/Ionization (MALDI)
- Purified peptide conjugation with DSPE-PEG for micelles synthesis
- Purified peptide conjugation with DSPE-PEG purification: High-Performance Liquid Chromoatography (HPLC)
- Purified peptide conjugation with DSPE-PEG purification: Matrix-Assisted Laser Desorption/Ionization (MALDI)
- Quantification of drug encapsulation in nanocarriers by HPLC
- siRNA conjugation with DSPE-PEG
Resource Development Core (RDC)
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Description
The Resource Development Core (RDC) offers a range of services including:
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Services
- Applications and use of magnetic ranking cytometry (LEAPFROG Platform)
- Cell surface target-specific phenotypic screening
- Cell surfaceome lentiviral CRISPR gRNA library
- Cell surfaceome library lentiviral prep
- Cell surfaceome library plasmid prep
- Cell surfaceome target detection optimization and characterization (LEAPFROG Platform)
- Cell-type specific antigen/target selection for magnetic ranking cytometry
- Cell-type specific target expression and distribution assessment
- Injection moulding based microfluidic chip fabriacation (LEAPFROG Platform)
- NGS data analysis (CRISPR gRNA enrichment assessment)
- Pooled CRISPR editing and phenotypic screening experimental designs
- Rare-cell isolation/enrichment using the LEAPFROG Platform
- Target-antibody-magnetic nanoparticles coupling/optimization
- Use of Cell Surfaceome lentiviral CRISPR gRNA library
- Use of injection moulded microfluidic chips for cell sorting (LEAPFROG platform)
- Use of microfluidc chips for cell sorting (LEAPFROG Platform)
Contact Information
If you would like more information regarding services provided by NUGoKidney, please contact:
Esmeralda Liz
nephroHUB@northwestern.edu
Website