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The George M. O’Brien Kidney Resource Alliance (OKRA) provides roughly 400 specialized resources, tools, technologies, services, and expertise beyond those typically available in an individual lab or institutional core. If you are interested in requesting any of the resources below, please click the “Request Here” button below. If you have any questions, email us at okrancc@uab.edu:

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CategoryResource Title Resource Center Core
Animal Models/Procedures Rodent cisplatin kidney injuryIndiana University Intravital core/Molecular Imaging Resource cores
Animal Models/Procedures Rodent Kidney Ischemia reperfusion injuryIndiana University Intravital core/Molecular Imaging Resource cores
Animal Models/Procedures Rodent NephrectomyIndiana University Intravital core/Molecular Imaging Resource cores
Animal Models/Procedures Rodent sepsis modelsIndiana University Intravital core/Molecular Imaging Resource cores
Animal Models/Procedures Comprehensive mouse kidney phenotyping for diet and stress mechanismsJohns Hopkins Biomedical Resource Core (B-DSSL)
Animal Models/Procedures Germ-free facility consultation useJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Germ-free sample banksJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures GFR measurementsJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Humanized mouse diet protocolsJohns Hopkins Biomedical Resource Core (B-DSSL)
Animal Models/Procedures Immunological manifestations of dietary and social stressors in the kidneyJohns Hopkins Biomedical Resource Core (B-DSSL)
Animal Models/Procedures Isolated Nephron segment Ex Vivo AnalysisJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Kidney Slice PreparationJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Metabolic Cage and Clearance StudiesJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Metabolism phenotypingJohns Hopkins Biomedical Resource Core (B-DSSL)
Animal Models/Procedures Mouse biobank of dietary and social stress modelsJohns Hopkins Biomedical Resource Core (B-DSSL)
Animal Models/Procedures Optical ClearingJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Plasma and Urine Electrolyte Panels Johns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Protocols, training, and service to isolate and characterize kidney immune cells by flow cytometric analysis (FACS)Johns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Small Sample RNAseq in Isolated Nephron SegmentsJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Telemetric Blood pressure MonitoringJohns Hopkins Biomedical Resource Core (B-DSSL) 
Animal Models/Procedures Model systems studies to identify pathways shared cross-speciesMichiganApplied Systems Biology Core 
Animal Models/Procedures Animal Handling and HusbandryPittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Blood pressure telemetryPittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Mitochondrial / Bioenergetics (Seahorse)PittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Purine metabolomicsPittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Injury biomarker assessmentPittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Measurement of GFR and RBFPittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Metabolic cage studies, urine metabolitesPittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Real-time super-resolution ultrasound of kidney microvasculaturePittsburghModel Systems Core 
Animal Models/Procedures Animal Models - Tissue HarvestingPittsburghModel Systems Core 
Animal Models/Procedures Disease Models: AKI (cecal ligation and puncture)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: AKI (cisplatin nephrotoxicity)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: AKI (gentamicin nephrotoxicity)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: AKI (ischemia-reperfusion injury)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: AKI (sickle cell crisis)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: CKD (aristolochic acid)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: CKD (chronic angiotensin II infusion)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: CKD (FSGS)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: CKD (genetic models)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: CKD (two kidney one clip hypertension)PittsburghModel Systems Core 
Animal Models/Procedures Disease Models: CKD (unilateral ureteral obstruction)PittsburghModel Systems Core 
Animal Models/Procedures Epithelial Models - Mammalian cell culture models (proximal tubule, collecting duct, polarized epithelia)PittsburghModel Systems Core 
Animal Models/Procedures Epithelial Models - Organoid models to study kidney injuryPittsburghModel Systems Core 
Animal Models/Procedures Epithelial Models - Organoid models to study kidney physiologyPittsburghModel Systems Core 
Animal Models/Procedures Epithelial Models - Organoid models to study nephron developmentPittsburghModel Systems Core 
Animal Models/Procedures Special Rodent Diets (low and high Na+ and K+)PittsburghModel Systems Core 
Animal Models/Procedures Yeast Models -  Positive genetic selection assaysPittsburghModel Systems Core 
Animal Models/Procedures Yeast Models -  Protein quality control (degradation, ubiquitylation) assaysPittsburghModel Systems Core 
Animal Models/Procedures Yeast Models - Bioinformatic analysis of genotype-phenotype correlations (TopMed, Clinvar, UK BioBank)PittsburghModel Systems Core 
Animal Models/Procedures Yeast Models - Computational identification of functional variantsPittsburghModel Systems Core 
Animal Models/Procedures Yeast Models - Protein trafficking assays (immunofluorescence, density centrifugation)PittsburghModel Systems Core 
Animal Models/Procedures 5/6th nephrectomy (both steps)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Aorta TXUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Automated tail-cuff blood pressure determination (each, 6 days)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Blodd/Urine CollectionUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Cannulation (indwelling with injection port)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Determination of GFR in awake rodentsUAB-UCSDPre-Clinical Core 
Animal Models/Procedures FistulaUAB-UCSDPre-Clinical Core 
Animal Models/Procedures GFR (transcutaneous FITC-sinistrin) in rodentsUAB-UCSDPre-Clinical Core 
Animal Models/Procedures IP InjectionUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Kidney ischemia/reperfusion (IRI)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Kidney TXUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Laser Capture Microdissection from frozen tissue (~70 pieces individually collected per sample; sample quality and amount appropriate for RNA-Seq or potentially proteomics)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Metabolic cage experiments in mice (per animal and week)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Micropuncture for nephron function and TG feedbackUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Misc. surgical services involving body wall penetration (per hour)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Orthotopic kidney transplantationUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Osmotic pump implantUAB-UCSDPre-Clinical Core 
Animal Models/Procedures ParabiosisUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Parabiosis (mice)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures Radio-telemetry determination of blood pressureUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Tail vein injection or venipuncture (per animal)UAB-UCSDPre-Clinical Core 
Animal Models/Procedures ThymectomyUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Tissue HarvestUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Tissue oxygen partial pressure determination in kidneyUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Unilateral Ureteral ObstructionUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Whole kidney clearance/oxygen consumption studiesUAB-UCSDPre-Clinical Core 
Animal Models/Procedures Analysis of VUS in non-COL4A genes through generation and analysis of gene edited modelsWUSTLVariant Validation Core 
Animal Models/Procedures COL4A3/A4/A5 VUS analysis in vivo by mouse gene editingWUSTLVariant Validation Core 
Animal Models/Procedures Generation of primary cells for Seahorse or stable isotope studiesWUSTLMetabolism Core
Animal Models/Procedures Stable isotope tracing studies in vivo: 15N-glutamine: data provisioningWUSTLMetabolism Core
Animal Models/Procedures Stable isotope tracing studies in vivo: branched chain amino acids: data provisioningWUSTLMetabolism Core
Biostatistics/BioinformaticsData analysis and visualizationIndiana University Intravital core/Molecular Imaging Resource cores
Biostatistics/BioinformaticsData cleaning, filtering and mappingIndiana University Molecular Imaging Resource core
Biostatistics/BioinformaticsBioinformatic AnalysisJohns Hopkins Biomedical Resource Core (B-DSSL) 
Biostatistics/BioinformaticsBiostatistical support (including clinical trials, epidemiological cohorts, case-control studies, survey data, and -omics studies)Johns Hopkins Biomedical Resource Core (C-DSSL) 
Biostatistics/BioinformaticsAnimal Transcript driven data-mining workflow - Bulk RNA Sequencing analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsAnimal Transcript driven data-mining workflow - Molecular pathway analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsAnimal Transcript driven data-mining workflow - Single cell/single nuc RNA Sequencing analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsCELLxGENE - data extraction service MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsHuman Transcript driven data-mining workflow - Bulk RNA Sequencing analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsHuman Transcript driven data-mining workflow - Molecular pathway analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsHuman Transcript driven data-mining workflow - Single cell/single nuc RNA Sequencing analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsMetabolomics data-mining workflow - Molecular pathway analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsMulti-scalar data analysis and integration, including cross-species comparison MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsNephroseq - data extraction service MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsProtein data-mining workflow - Molecular pathway analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsProtein data-mining workflow - proteomics data analysis pipeline MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsQuantitative morphometric analyses of kidney biopsy samples MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsTranSMART CProbe - data extraction service MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsTranSMART CureGN - data extraction service MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsTranSMART NEPTUNE - data extraction service MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsVitessce - data extraction service MichiganApplied Systems Biology Core
Biostatistics/BioinformaticsCELLxGENE - access/support MichiganData Analytic Services Core
Biostatistics/BioinformaticsDevelop generalized workflow for integrative web-based analysis MichiganData Analytic Services Core
Biostatistics/BioinformaticsInvestigating biomarker association (gene, protein, metabolite level) in clinical existing cohorts through TranSMART instance MichiganData Analytic Services Core
Biostatistics/BioinformaticsNephroseq - access/support MichiganData Analytic Services Core
Biostatistics/BioinformaticsTranSMART CProbe - access/support MichiganData Analytic Services Core
Biostatistics/BioinformaticsTranSMART CureGN - access/support MichiganData Analytic Services Core
Biostatistics/BioinformaticsTranSMART NEPTUNE - access/support MichiganData Analytic Services Core
Biostatistics/BioinformaticsVitessce - access/support MichiganData Analytic Services Core
Biostatistics/BioinformaticsData analysis - cellular and whole animal physiological studies, or imaging studiesPittsburghAll Cores 
Biostatistics/BioinformaticsAKI Study Analysis and InterpretationUAB-UCSDClinical Core 
Biostatistics/BioinformaticsAnalyses of study resultsUAB-UCSDClinical Core 
Biostatistics/BioinformaticsAnalysis of pilot dataUAB-UCSDClinical Core 
Biostatistics/BioinformaticsSample size and/or power analysisUAB-UCSDClinical Core 
Biostatistics/BioinformaticsBioinformatics and biostatistical supportWUSTLMetabolism Core
Biostatistics/BioinformaticsStable isotope flux studies- proximal tubules: 15N-glutamine: data provisioningWUSTLMetabolism Core
Biostatistics/BioinformaticsStable isotope tracing studies in vivo: 13C-glucose: data provisioningWUSTLMetabolism Core
Biostatistics/BioinformaticsStable isotope tracing studies in vivo: 13C-glutamine: data provisioningWUSTLMetabolism Core
Biostatistics/BioinformaticsStable isotope tracing studies in vivo: 13C-palmitate and 13C-oleate: data provisioningWUSTLMetabolism Core
Data ProvisioningDietary assessment services to investigate dietary patterns and biomarkers of dietary intake and food metabolismJohns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningGenomics dataset w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningKidney Precision Medicine Center of Excellence (KPMCOE)—EMR registryJohns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningLarge longitudinal cohort study data from racially and socioeconomically diverse populationsJohns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningMetabolomics dataset w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningProteomics dataset w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningPublicly accessible biospecimen repositories and datasets to support or conduct biomarker studies and secondary data analysesJohns Hopkins Biomedical Resource Core (C-DSSL) 
Data ProvisioningClinical and molecular interactions in investigators’ data setsMichiganApplied Systems Biology Core 
Data ProvisioningData ProvisioningPittsburghAll Cores
Data ProvisioningBig data transformation & pipeline development UAB-UCSDClinical Core 
Data ProvisioningCloud-based multimodal databases for AKI and critical care nephrology research studiesUAB-UCSDClinical Core 
Data ProvisioningDatabase developmentUAB-UCSDClinical Core 
Data ProvisioningStable isotope flux studies- proximal tubules: 15N-glutamine: data provisioningWUSTLMetabolism Core
Data ProvisioningStable isotope tracing studies in vivo: 13C-glucose: data provisioningWUSTLMetabolism Core
Data ProvisioningStable isotope tracing studies in vivo: 13C-glutamine: data provisioningWUSTLMetabolism Core
Data ProvisioningStable isotope tracing studies in vivo: 13C-palmitate and 13C-oleate: data provisioningWUSTLMetabolism Core
Laboratory ServicesMicrobiomeJohns Hopkins Biomedical Resource Core (B-DSSL)
Laboratory ServicesBiomarker measurement - Clinical Chemistry Johns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesBiomarker measurement - Immunoassays Johns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesBiorepository support - Processing aliquoting, short- and long-term sample storageJohns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesHealthy volunteer samples for Reference Range Johns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesPolymer synthesis for making nanoparticlesJohns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesProspective remnant sample collection Johns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesStored biospecimen samples from completed studies Johns Hopkins Biomedical Resource Core (C-DSSL) 
Laboratory ServicesStructured RNA-DNA sequence data integrationMichiganApplied Systems Biology Core 
Laboratory ServicesFluorophore conjugation with DSPE-PEGNU-USCBiomedical Resource Core
Laboratory ServicesIn vitro cell viability evaluation - MTS assay/plate readerNU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Binding Evaluation NU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Binding Evaluation: Confocal MicroscopyNU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Binding Evaluation: Flow CytrometryNU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Efficacy EvaluationNU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Efficacy: Quantification of markers related - qPCRNU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Efficacy: Quantification of proteins related - ELISANU-USCBiomedical Resource Core
Laboratory ServicesIn Vitro Micelles Efficacy: Quantification of proteins related - Western blotNU-USCBiomedical Resource Core
Laboratory ServicesIn vitro studies of micelles with Collecting Duct cells - Cortical collecting duct (M1)NU-USCBiomedical Resource Core
Laboratory ServicesIn vitro studies of micelles with Collecting Duct cells - Cortical collecting duct (Principal cells)NU-USCBiomedical Resource Core
Laboratory ServicesIn vitro studies of micelles with Collecting Duct cells - Inner Medullary NU-USCBiomedical Resource Core
Laboratory ServicesIn vitro studies of micelles with Proximal Tubule cellsNU-USCBiomedical Resource Core
Laboratory ServicesIn Vivo Micelles Therapeutic Efficacy: Immunohistochemistry (IHC) ImagingNU-USCBiomedical Resource Core
Laboratory ServicesIn Vivo Micelles Therapeutic Efficacy: using Rapid Model NU-USCBiomedical Resource Core
Laboratory ServicesIncorporation of conjugated siRNA into micellesNU-USCBiomedical Resource Core
Laboratory ServicesMicelles formationNU-USCBiomedical Resource Core
Laboratory ServicesNanocarrier/micelles characterization: Morphology - Transmission electron microscopy (TEM)NU-USCBiomedical Resource Core
Laboratory ServicesNanocarrier/micelles characterization: particles size - Dynamic Light Scattering (DLS) NU-USCBiomedical Resource Core
Laboratory ServicesNanocarrier/micelles characterization: Polydispersity Index (PDI)- Dynamic Light Scattering (DLS) NU-USCBiomedical Resource Core
Laboratory ServicesNanocarrier/micelles characterization: RNA incorporation - Gel ElectrophoresisNU-USCBiomedical Resource Core
Laboratory ServicesNanocarrier/micelles characterization: RNA protection against nucleases - Gel ElectrophoresisNU-USCBiomedical Resource Core
Laboratory ServicesNanocarrier/micelles characterization: zeta potential - Dynamic Light Scattering (DLS) NU-USCBiomedical Resource Core
Laboratory ServicesPeptide purificationNU-USCBiomedical Resource Core
Laboratory ServicesPeptide purification: High-Performance Liquid Chromatography (HPLC)NU-USCBiomedical Resource Core
Laboratory ServicesPeptide purification: Matrix-Assisted Laser Desorption/Ionization (MALDI)NU-USCBiomedical Resource Core
Laboratory ServicesPurified peptide conjugation with DSPE-PEG for micelles synthesisNU-USCBiomedical Resource Core
Laboratory ServicesPurified peptide conjugation with DSPE-PEG purification: High-Performance Liquid Chromoatography (HPLC)NU-USCBiomedical Resource Core
Laboratory ServicesPurified peptide conjugation with DSPE-PEG purification: Matrix-Assisted Laser Desorption/Ionization (MALDI)NU-USCBiomedical Resource Core
Laboratory ServicesQuantification of drug encapsulation in nanocarriers by HPLCNU-USCBiomedical Resource Core
Laboratory ServicessiRNA conjugation with DSPE-PEGNU-USCBiomedical Resource Core
Laboratory ServicesFunctional imaging analysis (e.g., Ca2+ imaging)PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Laboratory ServicesHPLC-Fl - ATP MetabolomePittsburghModel Systems Core
Laboratory ServicesPurine Metabolome by UPLC-MS/MSPittsburghModel Systems Core
Laboratory ServicesEpithelial transport in Ussing chambersPittsburghPhysiology Core 
Laboratory ServicesFunctional imaging analysis (e.g., Ca2+ imaging)PittsburghPhysiology Core
Laboratory ServicesIn vitro microperfusion of isolated tubules for functional fluorescence assays of single cell functionPittsburghPhysiology Core
Laboratory ServicesIn vitro microperfusion of isolated tubules for measurement of transepithelial ion and solute transportPittsburghPhysiology Core 
Laboratory ServicesIsolation of single nephron segments for immunofluorescence, enzyme assays, RNA and protein isolationPittsburghPhysiology Core 
Laboratory ServicesIsolation of Xenopus laevis oocytesPittsburghPhysiology Core 
Laboratory ServicesOocyte two-electrode voltage clampPittsburghPhysiology Core 
Laboratory ServicesPatch-clamp analysesPittsburghPhysiology Core 
Laboratory ServicesQuantification of surface protein expressionPittsburghPhysiology Core 
Laboratory ServicesAKI Biological Sample RepositoryUAB-UCSDClinical Core 
Laboratory ServicesBulk RNA seq - from Fastq to analyses UAB-UCSDPre-Clinical Core 
Laboratory ServicesBulk RNA seq - sample to analyses UAB-UCSDPre-Clinical Core 
Laboratory ServicesLC-MS/MS Creatinine determination (per test)UAB-UCSDPre-Clinical Core 
Laboratory ServicesUse of a microsurgical workstation (per hour)UAB-UCSDPre-Clinical Core 
Laboratory ServicesBCA Assay for normalization of fatty acid oxidation dataWUSTLMetabolism Core
Laboratory ServicesBranched chain amino acid oxidation in tissue ex vivo using 14C-KIVAWUSTLMetabolism Core
Laboratory ServicesCell quantification with Biotek Lionheart (to normalize Seahorse data)WUSTLMetabolism Core
Laboratory ServicesFatty acid oxidation in tissue ex vivo using 3H-palmitateWUSTLMetabolism Core
Laboratory ServicesGlucose oxidation in tissue ex vivo using 14C-pyruvateWUSTLMetabolism Core
Laboratory ServicesPlacement of jugular catheter for administration of stable isotopesWUSTLMetabolism Core
Laboratory ServicesSeahorse bioflux analyzer (XF24/XF96) to measure glucose oxidation in primary proximal tubule cellsWUSTLMetabolism Core
Laboratory ServicesSeahorse bioflux analyzer (XF24/XF96) to measure glutamine oxidation in primary proximal tubule cellsWUSTLMetabolism Core
Laboratory ServicesSeahorse bioflux analyzer (XF24/XF96) to measure glycolysis (i.e. extracellular acidification rate) in primary proximal tubule cellsWUSTLMetabolism Core
Laboratory ServicesSeahorse bioflux analyzer (XF24/XF96) to measure mitochondrial oxidative respiration in primary proximal tubule cellsWUSTLMetabolism Core
Laboratory ServicesSeahorse bioflux analyzer (XF24/XF96) to measure palmitate (fatty acid) oxidation in primary proximal tubule cellsWUSTLMetabolism Core
Laboratory ServicesStable isotope flux studies- proximal tubules: 13C-glucoseWUSTLMetabolism Core
Laboratory ServicesStable isotope flux studies- proximal tubules: 13C-glutamineWUSTLMetabolism Core
Laboratory ServicesStable isotope flux studies- proximal tubules: 13C-palmitateWUSTLMetabolism Core
Laboratory ServicesStable isotope tracing studies in vivo: 13C-glucoseWUSTLMetabolism Core
Laboratory ServicesStable isotope tracing studies in vivo: 13C-glutamineWUSTLMetabolism Core
Laboratory ServicesStable isotope tracing studies in vivo: 13C-palmitate and 13C-oleateWUSTLMetabolism Core
Laboratory ServicesStable isotope tracing studies in vivo: 15N-glutamineWUSTLMetabolism Core
Laboratory ServicesStable isotope tracing studies in vivo: branched chain amino acidsWUSTLMetabolism Core
Laboratory ServicesUntargeted metabolomics - lipidsWUSTLMetabolism Core
Laboratory ServicesUntargeted metabolomics - water solubleWUSTLMetabolism Core
Laboratory ServicesAnalysis of  variant protein trafficking in cultured cellsWUSTLVariant Validation Core 
Laboratory ServicesAnalysis of variant UMOD protein trafficking in cultured cellsWUSTLVariant Validation Core 
Laboratory ServicesAnalysis of VUS in non-COL4A genes through design and implementation of tailored laboratory assaysWUSTLVariant Validation Core 
Laboratory ServicesCOL4A3 intronic VUS analysis by minigene RNA splicing assays using transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A3 VUS (missense) analysis by N-terminal split nano-Luciferase collagen IV assembly and secretion assays in transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A3 VUS (missense) analysis by split C-terminal nano-Luciferase collagen IV assembly and secretion assays using transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A3 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsiesWUSTLVariant Validation Core 
Laboratory ServicesCOL4A4 intronic VUS analysis by minigene RNA splicing assays using transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A4 VUS (missense) analysis by N-terminal split nano-Luciferase collagen IV assembly and secretion assays in transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A4 VUS (missense) analysis by split C-terminal nano-Luciferase collagen IV assembly and secretion assays using transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A4 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsiesWUSTLVariant Validation Core 
Laboratory ServicesCOL4A5 intronic VUS analysis by minigene RNA splicing assays using transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A5 VUS (missense) analysis by N-terminal split nano-Luciferase collagen IV assembly and secretion assays in transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A5 VUS (missense) analysis by split C-terminal nano-Luciferase collagen IV assembly and secretion assays using transfected cellsWUSTLVariant Validation Core 
Laboratory ServicesCOL4A5 VUS analysis by high resolution quantitative immunofluorescence imaging in paraffin sections of kidney biopsiesWUSTLVariant Validation Core 
Laboratory ServicesIntronic non-COL4A gene VUS analysis by minigene RNA splicing assays using transfected cells WUSTLVariant Validation Core 
Methods DevelopmentMethods development in microscopy and molecular imagingIndiana University Intravital core/Molecular Imaging Resource cores
Methods DevelopmentGeneral data result interpretation and discussion, development of next stepsMichiganApplied Systems Biology Core
Methods DevelopmentGeneral troubleshootingMichiganApplied Systems Biology Core
Methods DevelopmentInitial study design consultation MichiganApplied Systems Biology Core
Methods DevelopmentMetabolomics driven analysis workflow MichiganApplied Systems Biology Core
Methods DevelopmentSpatial metabolomics service (sample process and data analysis) MichiganApplied Systems Biology Core
Methods DevelopmentTranscript driven data-mining workflow - Bulk RNA Sequencing analysis pipeline MichiganApplied Systems Biology Core
Methods DevelopmentTranscript driven data-mining workflow - Single cell/single nuc RNA Sequencing analysis pipeline MichiganApplied Systems Biology Core
Methods DevelopmentTranscript driven data-mining workflow - Streamlined self-organizing map (SOM) to identify functional subgroups MichiganApplied Systems Biology Core
Methods DevelopmentTranscript driven data-mining workflow - Weighted Correlation Network Analysis (WGCNA) to identify co-expressed molecular elements within datasets MichiganApplied Systems Biology Core
Methods DevelopmentDesign of epidemiological, clinical studies or clinical trialsUAB-UCSDClinical Core 
Methods DevelopmentForms, manual of operations, and/or instrument developmentUAB-UCSDClinical Core 
Methods DevelopmentMethodologies and Applications of Artificial Intelligence and Digital HealthUAB-UCSDClinical Core 
Methods DevelopmentResearch Resource Development (insert link for Development Core)UAB-UCSDResource Development Core 
Methods DevelopmentDesign of assays for other protein trafficking in cultured cellsWUSTLVariant Validation Core 
Microscopy/Imaging ServicesCharacterization of transgenic rodents expressing fluorescently tagged proteins in the kidneyIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesFluorescence lifetime imaging of endogenous and exogenous fluorophores in the kidneyIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Apoptosis and necrosisIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Cellular oxidative stressIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Characterization of in vivo fluorescent probe delivery to the kidneyIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Glomerular PermeabilityIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Microvascular flowIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Microvascular permeabilityIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Mitochondrial functionIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging ServicesIntravital Imaging: Proximal tubule endocytosisIndiana University Intravital Microscopy Resource Core 
Microscopy/Imaging Services3-dimensional confocal tissue cytometry of kidney tissueIndiana University Molecular Imaging Resource Core 
Microscopy/Imaging ServicesCODEX multiplexed cytometry of mouse and human kidney tissue, other tissues/organsIndiana University Molecular Imaging Resource Core 
Microscopy/Imaging ServicesIn situ sequencing of mouse and human kidney tissue (Xenium)Indiana University Molecular Imaging Resource Core 
Microscopy/Imaging ServicesLightsheet microscopyIndiana University Molecular Imaging Resource Core 
Microscopy/Imaging ServicesSpatial Transcriptomics of mouse and human kidney tissue (Visium SD, Visium FFPE)Indiana University Molecular Imaging Resource Core 
Microscopy/Imaging ServicesImages w/ deep phenotyping Johns Hopkins Biomedical Resource Core (C-DSSL) 
Microscopy/Imaging ServicesHistopathologyPittsburghAll Cores
Microscopy/Imaging ServicesConfocal microscopy - Laser scanning confocal microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesConfocal microscopy - Spinning disk confocal microscopy PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesConfocal microscropy - Fluorescent lifetime imagingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesConfocal microscropy - Fluorescent recovery after photobleachingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesConfocal microscropy - Ribbon scanning confocal microscopy for cleared organs/tissuesPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - CLEMPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Freeze fracture microscopy PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Freeze substitutionPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Immuno-Electron microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Negative stainingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Platinum replica electron microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Scanning electron microscopy (field-emission)PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Transmission electron microscopy PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - UltramicrotomyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesElectron Microscopy - Ultrathin cryo EMPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesImage Analysis - ImarisPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesImage Analysis - Leica LASX PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesImage Analysis - MetamorphPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesImage Analysis - NIH image/FIJIPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesImage Analysis - NIS elementsPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesImage Analysis - PhotoshopPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesiPOL imagingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesLive-cell microscopy - Fluorescent lifetime imagingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesLive-cell microscopy - Fluorescent recovery after photobleachingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesLive-cell microscopy - Incubator microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesLive-cell microscopy - Lattice light sheet microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesLive-cell microscopy - Spinning disk confocal microscopy PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesLive-cell microscopy -MicroinjectionPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesMeso-Spim light sheet microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesMultiphoton microscopy - Fluorescent lifetime imagingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesMultiphoton microscopy - Fluorescent recovery after photobleachingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesMultiphoton microscopy - Intravital imagingPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesMultiphoton microscopy - Multiphoton confocal microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesStandard light microscopy - Brightfield, darkfield, epifluorescence, DICPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesStandard light microscopy - Macro (stereo) imaging microscopyPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesSuper-resolution microscopy - AX/NSPARCPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesSuper-resolution microscopy - CRESTPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesSuper-resolution microscopy - STEDPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesSuper-resolution microscopy - STORMPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesTRIF Microscopy - TIRF imaging acquisition and analysisPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesWhole slide scanning - brightfield and epifluorescence PittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Microscopy/Imaging ServicesFunctional imaging analysesPittsburghPhysiology Core 
Microscopy/Imaging ServicesFunctional renal imaging (tracer dependent, per hour)UAB-UCSDPre-Clinical Core 
Microscopy/Imaging ServicesImage AnalysisUAB-UCSDPre-Clinical Core 
Microscopy/Imaging ServicesNuclear imaging (Gamma camera, SPECT/CT, PET/CT) (per hour)UAB-UCSDPre-Clinical Core 
Microscopy/Imaging ServicesOptical Imaging (Bioluminescence, Fluorescence) (per hour)UAB-UCSDPre-Clinical Core 
Microscopy/Imaging ServicesStructural and metabolic imaging (US, MRI, microCT) (per hour)UAB-UCSDPre-Clinical Core 
Project ConsultationAKI study design and planning for imaging studiesIndiana University Intravital core/Molecular Imaging Resource cores
Project ConsultationCKD study design and planning for imaging studiesIndiana University Intravital core/Molecular Imaging Resource cores
Project ConsultationProject consultation for imaging studiesIndiana University Intravital core/Molecular Imaging Resource cores
Project ConsultationInitial project consultation to select appropriate Basic Science Dietary and Social Stressor Laboratory resourcesJohns Hopkins Biomedical Resource Core (B-DSSL) 
Project ConsultationStudy design, Basic Science Dietary and Social Stressor LaboratoryJohns Hopkins Biomedical Resource Core (B-DSSL) 
Project ConsultationConsultation on design and features of translational studies in the communityJohns Hopkins Biomedical Resource Core (C-DSSL) 
Project ConsultationInitial project consultation to select appropriate Clinical Science Dietary and Social Stressor Laboratory resourcesJohns Hopkins Biomedical Resource Core (C-DSSL) 
Project ConsultationStudy design consultation and data and biobanked specimens from studies on behavioral interventions for lifestyle changesJohns Hopkins Biomedical Resource Core (C-DSSL) 
Project ConsultationStudy design, Clinical Science Dietary and Social Stressor LaboratoryJohns Hopkins Biomedical Resource Core (C-DSSL) 
Project ConsultationShared Data Mining Services - Initial study design consultation MichiganApplied Systems Biology Core 
Project ConsultationApplications of immunomagnetic cell sorting (LEAPFROG Platform)NU-USCBiomedical Resource Core
Project ConsultationDesigning in vitro studiesNU-USCBiomedical Resource Core
Project ConsultationDesigning in vivo studiesNU-USCBiomedical Resource Core
Project ConsultationFeasibility assessmentNU-USCBiomedical Resource Core
Project ConsultationIdentifying pathway for therapyNU-USCBiomedical Resource Core
Project ConsultationIdentifying targeting peptidesNU-USCBiomedical Resource Core
Project ConsultationIn Vivo Micelles Therapeutic Efficacy : Hematoxylin and Eosin (H&E) ImagingNU-USCBiomedical Resource Core
Project ConsultationIn Vivo Therapeutic Efficacy: NU-USCBiomedical Resource Core
Project ConsultationIn Vivo Therapeutic Efficacy: GuidanceNU-USCBiomedical Resource Core
Project ConsultationNW Background searchNU-USCBiomedical Resource Core
Project ConsultationNW Grant proposal assistanceNU-USCBiomedical Resource Core
Project ConsultationConsultation for development of new approaches to study the kidneys and lower urinary tractPittsburghAll Cores
Project ConsultationAKI Clinical Study Initiation and ConductUAB-UCSDClinical Core 
Project ConsultationAKI Epidemiological Study Initiation and Conduct UAB-UCSDClinical Core 
Project ConsultationAKI Study Planning and DesignUAB-UCSDClinical Core 
Project ConsultationData Safety and MonitoringUAB-UCSDClinical Core 
Project ConsultationBulk RNA seq UAB-UCSDPre-Clinical Core 
Project ConsultationChoosing the right metabolic assayWUSTLMetabolism Core
Project ConsultationHigh resolution respirometry (Oroboros) WUSTLMetabolism Core
Project ConsultationMetabolomics and stable isotope flux study- Zoom discussion with Patti lab over experimental designWUSTLMetabolism Core
Project ConsultationChoosing an assay for COL4A3 VUS analysisWUSTLVariant Validation Core 
Project ConsultationChoosing an assay for COL4A4 VUS analysisWUSTLVariant Validation Core 
Project ConsultationChoosing an assay for COL4A5 VUS analysisWUSTLVariant Validation Core 
Project ConsultationChoosing an assay for non-COL4A gene VUS analysisWUSTLVariant Validation Core 
Publication SupportGrant AssistanceIndiana University Intravital core/Molecular Imaging Resource cores
Publication SupportManuscript development/publication supportIndiana University Intravital core/Molecular Imaging Resource cores
Publication SupportHopkins Manuscript developmentJohns Hopkins Biomedical Resources Core
Publication SupportConference, meeting abstract preparation MichiganAll cores
Publication SupportConference, meeting presentation support MichiganAll cores
Publication SupportGrant support - LOS MichiganAll cores
Publication SupportGrant support - Writing MichiganAll cores
Publication SupportMichigan Manuscript development MichiganAll cores
Publication SupportNU-USC Manuscript assistanceNU-USCBiomedical Resource Core
Publication SupportNU-USC Publication AssistanceNU-USCBiomedical Resource Core
Publication SupportManuscript developmentPittsburghAll Cores 
Publication SupportAssistance with manuscript development or publication supportUAB-UCSDAll Cores
Publication SupportAssistance with manuscript development or publication supportWUSTLMetabolism Core
Training/DevelopmentTraining - Includes Bioinformatics and Digital Image AnalysisIndiana University Intravital core/Molecular Imaging Resource cores
Training/DevelopmentTraining in Intravital microscopy Indiana University Intravital Resource core
Training/DevelopmentIsolated Nephron segment Dissection Johns Hopkins Biomedical Resource Core (B-DSSL) 
Training/DevelopmentKidney Slice Preparation  Johns Hopkins Biomedical Resource Core (B-DSSL) 
Training/DevelopmentCELLxGENE - training MichiganApplied Systems Biology Core
Training/DevelopmentGeneral bioinformatic tool training (e.g. R) MichiganApplied Systems Biology Core
Training/DevelopmentNephroSeq training MichiganApplied Systems Biology Core
Training/DevelopmentTranscript driven data analysis - Bulk RNA sequencing analysis pipeline MichiganApplied Systems Biology Core
Training/DevelopmentTranscript driven data analysis - Molecular pathway analysis pipeline MichiganApplied Systems Biology Core
Training/DevelopmentTranSMART CProbe - training MichiganApplied Systems Biology Core
Training/DevelopmentTranSMART CureGN - training MichiganApplied Systems Biology Core
Training/DevelopmentTranSMART NEPTUNE- training MichiganApplied Systems Biology Core
Training/DevelopmentVitessce - training MichiganApplied Systems Biology Core
Training/DevelopmentUse of injection moulded microfluidic chips for cell sorting (LEAPFROG platform)NU-USCResource Development Core
Training/DevelopmentTraining in live-cell and intravital approaches to study the biology/pathobiology of kidney and bladder tissuesPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training/DevelopmentTraining in microscope/equipment usagePittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training/DevelopmentTraining in quantitative image analysisPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training/DevelopmentTraining in sample preparation of kidney and bladder tissues for light and electron microscopic analysisPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training/DevelopmentTraining in specialized sample preparation to perform labeling and analysis of cleared organs and tissuesPittsburghKidney Imaging: Developing Novel and Innovative Tools (KIDNIT) Development Core
Training/DevelopmentNephron segment microdissection/isolation and microperfusion training PittsburghPhysiology Core 
Training/DevelopmentPatch-clamp training PittsburghPhysiology Core 
Training/DevelopmentTraining on use of microinjectorsPittsburghPhysiology Core 
Training/DevelopmentTraining on use of micropipette pullers and microforges to craft micropipettes for patch-clamp and in vitro microperfusionPittsburghPhysiology Core 
Training/DevelopmentTwo-electrode voltage clamp training PittsburghPhysiology Core 
Training/DevelopmentXenopus laevis technologies training PittsburghPhysiology Core 
Training/DevelopmentBulk RNA seq training  (per day)UAB-UCSDPre-Clinical Core 
Training/DevelopmentTraining (per hour/per day)UAB-UCSDPre-Clinical Core 
Training/DevelopmentTraining in the performance of collagen IV heterotrimerization assays using split nanlLuciferase tagged COL4A chains WUSTLVariant Validation Core 

 

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