The UAB Heersink School of Medicine Immunology Institute and the O’Neal Comprehensive Cancer Center (OCCC), working in close partnership with the Flow Cytometry and Single Cell (FCSC) core, have established human IMMUNOPHENOTYPING services to all members of the Immunology Institute and the UAB community.
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What is immunophenotyping?
What is immunophenotyping?
Immunophenotyping is the identification and quantitation of heterogeneous populations of cells by multi-parameter flow cytometry using a panel of fluorescently-labelled antibodies that recognize specific antigens on a cell surface, known as cell markers.
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Why use immunophenotyping in your work?
Why use immunophenotyping in your work?
Immunophenotyping is used to monitor the molecular, metabolic, phenotypic and functional attributes of cells that circulate through the blood. Alterations in these cellular attributes, particularly over time, may be closely associated with specific disease manifestations, disease progression and responsiveness to treatment. Thus, immunophenotyping is a powerful approach to identify easily monitored biomarkers and cellular signatures that may be useful as diagnostic and prognostic indicators of disease. Immunophenotyping is particularly helpful when monitoring patients who are exposed to immunotherapies, such as checkpoint inhibitors and targeted immune modulators that are increasingly used in chronic disease settings.
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Which immunophenotyping panels are available?
Which immunophenotyping panels are available?
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Composition of our validated PBMC, B cell and T cell panels
Composition of our validated PBMC, B cell and T cell panels
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Identification of 44 human immune cell subsets using the PBMC panel
Identification of 44 human immune cell subsets using the PBMC panel
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Identification of 23+ human B cell subpopulations using the B cell panel
Identification of 23+ human B cell subpopulations using the B cell panel
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Identification of 20 human T cell subpopulations using the BASIC T Cell panel
Identification of 20 human T cell subpopulations using the BASIC T Cell panel
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Identification of T cell subpopulations using the T cell panel
Identification of T cell subpopulations using the T cell panel
The T cell panel will enable the identification of:
- Effector CD4+ and CD8+ T cells
- Memory CD4+ and CD8+ T cells
- Exhausted CD4+ and CD8+ T cells
- Regulatory T cells
- Follicular helper T cells
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Point of contact
Point of contact
Davide Botta, PhD
Research Manager
HSOM Immunology Institute
dbotta@uab.edu
SHEL 575