• Thermo Fisher Orbitrap Velos Pro

    Dedicated to Leon Edwards of "Edwards Chevrolet" for his generous donation leading to the purchase of this instrument for cancer research

     LeonEdwardsEditedOrbitrapVelosPro



    Thermo Fisher Orbitrap Velos Pro

    Agilent 1260 infinity nano-HPLC stack and Auto-Sampler

    ThermoAgilent



    Thermo Fisher LTQ-XL

    Dionex Ultimate 3000 UHPLC+ Focused Stack & Auto Sampler

    ThermoDionex


    Thermo Fisher LTQ-XL with ETD

    Surveyor MS HPLC Stack & Auto-Sampler

    thermosurveyor


    Bruker UltraFlex III MALDI ToF/ToF

    bruker


    Shimadzu ChIP-1000 (HD Chemical Printer for Imaging-MS)

    Shimadzu


    GE Ettan™ IPGphor IEF System

    Large Format BioRad Protean® Plus Dodeca Cell w/ Power Supply

    Amersham Multitemp III w/ Circulating Pump

    GEEttanBioRadAmersham

    GE Ettan™ Spot Picker

    GEEttan


    BioTek Microplate Spectrophotometer

    (Synergy 2 Multi-Mode UV/ VIS/ / NIR/ FL/ L Top&Bottom Reader)

    BioTek


    UVP BioSpectrum® Imaging System (UV/ VIS/ IR/ FL/ L) 

     UVP


    Shandon Cryotome™ FSE Cryostat w/ Histology Station 

     Shandon


    Sutton Nano Laser Quartz Tip Puller with Column Packing Bomb 

     Sutton


    GE nanoCOLLECT System (LC-MALDI-MS Spotter)

    Surveyor MS HPLC Stack & Auto-sampler

     GESurveyor


    Shimadzu (analytical/ preparative) HPLC w-UV/Vis Detector

    Gilson Fraction Collector

     ShimadzuGilson


    Cell Culture Room: Laminar Flow Hood, CO2 Incubator, & 2 Microscopes

     Cell Culture Room


    Two -80°CRefrigerators (Long-Term Storage Available For MSP-SF Users)

     Two 80C Refrigerators


    Server Stack

     ServerStack


    Our facility houses nearly 30 computers on the UAB network in our own subdomain. Our computing equipment includes 2 high-end workstations and 5 high-speed high capacity double firewalled servers(below) that contains over 80TB of dynamic drive space and an additional 105TB of backup storage for archiving and automated nightly backups for all computers throughout the MSP-SF (including all MS instruments).

    • Primary fileserver: (44TB of drive space, 64 GB RAM,  AMD EPYC 7401P 24Core processors)
    • Unix application server: powered by an Oracle Database (64 GB RAM, 2 - AMD 2.6GHz 12 Core processors)
    • Primary domain controller: for our network (2 GB RAM, 1 - Intel 2.0 GHZ Dual Core processor)
    • Secondary fileserver: (30TB, 32 GB RAM , 2-AMD 3.1GHz 8 Core processors)
    • Application server: (64GB RAM, AMD EPYC 7401P 24Core processor 2.0GHz, 44TB between 1.7 TB high speed SSD and 6 8TB HDD in RAID 5 Array)
    • Backup Server: (6 Core Xeon Processor 1.9 GHz 8GB RAM 105TB Storage)
    • Virtual backup domain controlller

    The primary fileserver is also dynamically linked to a 2000 node Cluster - UAB Shared High Performance Computing Facility. (https://docs.uabgrid.uab.edu/wiki/Resources)

    Software:

    • Search Engines: SEQUEST, MASCOT
    • Denovo Sequencing: MASCOT Distiller
    • 2D PAGE Analysis: Nonlinear Samespots
    • MS Peptide & PTM Grouping, Filtering, and Quantification Analysis: MASCOT Distiller Q+, Proteome Software Scaffold, Scaffold PTM/ Q+S
    • Bruker Daltronics Suite: LCWARP, Biotools, Rapid Denovo, Fleximaging
    • Statistical Analysis: Matlab, Statistica, SPSS, Graphpad, Origin
    • Systems Analysis: GeneGO-metaCore, Blast2Go-Pro
    • Data Sharing: MSP-SF LIMS, all data is retrievable via HTML

    Additional Essential Equipment:

    • Small Molecule & Lipid Preparation Station (including two multi-well SPE devices w/ multi-well heated drying system – w/ argon & nitrogen)
    • Chemical Synthesis & Distillation Apparatus
    • Savant Explorer SpeedVac® Concentrator with multiple adapters including 96 deep well
    • Sorvall Legend XTR centrifuge with multiple adapters including a 96 deep well plate holder
    • Chemical Fume Hoods (2)
    • Refrigerated Cold Cabinets (2)
    • Freezers -20°C (2)
    • Multiple Centrifuges (cooled and RT)
    • Multiple Standard and Midi gel boxes for 1D PAGE & WB
    • 1D Gel Electro-Eluter
    • Thermocyclers (3)
    • High-Speed 96 Multi-Well Shaker
    • Heated Multi-Well Shaker
    • Large Water Bath Sonicator
    • Cell Disruptor